Extended Data Figure 3: Validation of enhancer screen. | Nature

Extended Data Figure 3: Validation of enhancer screen.

From: BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis

Extended Data Figure 3

a, HbF+ fraction in HUDEP-2 cells transduced in arrayed format with 24 sgRNAs from all 5 mapping categories with enrichment scores ranging from the highest to the lowest in the screen. b, Correlation between HbF enrichment score from pooled sgRNA screen and HbF+ fraction by arrayed validation of individual sgRNAs in HUDEP-2 cells. c, Erythroid differentiation of primary human erythroid precursors evaluated by CD71 and CD235a surface markers, enucleation frequency (CD235a+ Hoescht33342), and morphology by May–Grünwald Giemsa staining.

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