Extended Data Figure 9: Evaluation of the m+62 functional core. | Nature

Extended Data Figure 9: Evaluation of the m+62 functional core.

From: BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis

Extended Data Figure 9

200 bp at the functional core of the m+62 orthologue defined by HMM state. Enrichment scores are shown as grey lines and circles with the blue line representing smoothed enrichment score. JASPAR motifs (P < 10−4) are depicted with selected motifs annotated by transcription factor name on the basis of known erythroid-specific function or genomic position. Orthologous human sequences are listed. PhyloP (scale from −3.3 to 2.1) and PhastCons (from 0 to 1) estimates of evolutionary conservation among 30 vertebrates are shown. Individual numbered hemizygous deletion clones with indicated breakpoints were evaluated by BCL11A immunoblot (C, control). Clones 9 and 10 encompass the entire m+62 orthologue.

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