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STAP cells are derived from ES cells

Nature volume 525, pages E4–E5 (2015)Cite this article

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arising from H. Obokata et al. Nature 505, 641–647 (2014) doi:10.1038/nature12968; retraction 511, 112 (2014) doi:10.1038/nature13598 ; and H. Obokata et al. Nature 505, 676–680 (2014) doi:10.1038/nature12969; retraction 511, 112 (2014) doi:10.1038/nature13599

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Figure 1: STAP cells and STAP stem cells are derived from ES cells.

References

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Author information

Author notes

  1. Daijiro Konno, Takeya Kasukawa and Kosuke Hashimoto: These authors contributed equally to this work.

Authors and Affiliations

  1. Cell Asymmetry, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-Minamimachi, Chuo-ku, 650-0047, Kobe, Japan

    Daijiro Konno, Taeko Suetsugu & Fumio Matsuzaki

  2. Division of Genomic Technologies, RIKEN Center for Life Science Technologies, RIKEN Yokohama Campus, Yokohama, 230-0045, Kanagawa, Japan

    Takeya Kasukawa, Kosuke Hashimoto & Piero Carninci

  3. Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8503, Kanagawa, Japan

    Takehiko Itoh

  4. Japan Mouse Clinic, RIKEN BioResource Center, 3-1-1, Koyadai, Tsukuba-shi, 305-0074, Ibaraki, Japan

    Ikuo Miura & Shigeharu Wakana

Authors
  1. Daijiro Konno
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  2. Takeya Kasukawa
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  3. Kosuke Hashimoto
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  4. Takehiko Itoh
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  5. Taeko Suetsugu
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  7. Shigeharu Wakana
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  8. Piero Carninci
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  9. Fumio Matsuzaki
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Contributions

D.K., T.K. and H.H. all contributed equally to this work. D.K. designed and performed PCR analyses of genomic DNA from cell and tissue samples. T.K. and K.H. designed WGS and analysed the data. T.I. performed detailed whole-genome SNP analysis. T.S. histochemically analysed teratoma samples. E.M and S.W. performed TaqMan PCR analyses. P.C. designed and managed WGS and analysis. F.M. designed and organized this investigation, and wrote the manuscript.

Corresponding authors

Correspondence to Piero Carninci or Fumio Matsuzaki.

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Competing Financial Interests Declared none.

Extended data figures and tables

Extended Data Figure 1 Genome-wide SNP patterns of STAP-cell-related cells and mice.

a, SNP patterns of STAP stem cells, Fgf4-induced stem cells and related ES cells as revealed by WGS. Chromosomes 1–19 and X are aligned from left to right. All cell lines and mouse strains except for STAP stem cell GLS1, GOF-ES and GOF-mouse are male. 129/GFP ES cells and the re-sequenced control DNA of STAP cells for ChIP-seq (Fig. 4 in ref. 2) are also shown. B6-homozygous, B6/129-heterozygous and 129-homozygous SNPs are shown in magenta, green and blue, respectively. Note that ntESG1 and ntESG2 inherited the B6-type X chromosome from maternal mice. Genomic regions in which FES1 and FES2 ES cells have different SNP clusters in chromosomes (chromosomes 6, 11 and 12) are marked by red rectangles. See b, c and Fig. 1b for a high-resolution map. SNP resolution is 10 Mb. b, c, High-resolution view of chromosomes 6 (b) and 11 (c), which show differential SNP clusters (red rectangles) between FES1 and FES2. In these regions, all SNPs are 129-type in FES1, and B6-type in FES2 on one of the homologous chromosomes. Therefore, in these regions, 129/B6 SNPs (green) and 129/129 SNPs (blue) in FES1 correspond to B6/B6 SNPs (red) and B6/129s (green) in FES2. STAP stem-cell line FLS3, Fgf4-induced stem-cell line CTS1, and 129/GFP ES cells share the same SNP patterns with FES1. SNP resolution is 1 Mb.

Extended Data Figure 2 STAP stem cells derived from GOF-ES cells and cag-GFP ES cells.

a, Copy number of chromosome X in STAP stem cells GLS1 and GOF-ES cells, both of which have Oct4-gfp transgenes with a B6 background. These lines have one very short X chromosome of ∼23 Mb with a terminal inverted repeat and a normal X chromosome. b, PCR detection of chromosomal anomalies and Y chromosome in cag-GFP STAP stem-cell lines and parental mouse strains. Lanes 1–6: control ES cells, 129B6F1 ES1–6; 7: STAP stem cell AC129-1; 8: STAP stem cell AC129-2; 9: STAP stem cell FLS-T1; 10: STAP stem cell FLS-T2; and 11: GOF-ES. Deletions 1–4 and duplication 1 are located on Chr19: 32,857093–32866,121, Chr1:140,698,249–140,702,693, Chr4:123,747,239–123,763,596, Chr10:43,265,147–43,267,270 and Chr1: 180,730,393–180,732,937, respectively. c, Distribution of B6-type and 129-type SNPs along chromosome 6. The B6-homozygous SNP cluster (magenta) in the middle, which probably arose from the inheritance of the parental 129, is heterogeneous in length among six control ES cell lines. The four cag-GFP STAP stem-cell lines share the same length of the B6 SNP cluster with control ES 129B6F1 ES1. Note that the 129/B6-heterozygous SNP region in the 129 cag-GFP mouse is longer than that of AC129-1. d, Table summarizing the chromosomal anomalies and differential types of Chr6 B6-homozogous SNP clusters in the cag-GFP cell lines and parental mice. Control ES cell 129B6F1 ES1 shares all the characteristic features with the four cag-GFP STAP stem-cell lines.

Extended Data Table 1 STAP-cell-related cell lines
Full size table

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Konno, D., Kasukawa, T., Hashimoto, K. et al. STAP cells are derived from ES cells. Nature 525, E4–E5 (2015). https://doi.org/10.1038/nature15366

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