Extended Data Figure 10: Expression analysis of Wnt-associated genes in primary AML patient samples. | Nature

Extended Data Figure 10: Expression analysis of Wnt-associated genes in primary AML patient samples.

From: Transcriptional plasticity promotes primary and acquired resistance to BET inhibition

Extended Data Figure 10

a, Determination of JQ1 response profiles in 12 primary AML patient samples. Sensitivity was determined using 3H-thymidine uptake across different JQ1 concentrations. b, qRT–PCR analysis of mRNA levels of additional Wnt-associated genes in primary human leukaemia samples relative to GAPDH (continuation of Fig. 4g). Patient groups with low JQ1 IC50 (<200 nM, blue dots) were compared to patients with high IC50 (>500 nM, red dots). Statistical significance was determined using a Student’s t-test. c, Definition of a JQ1 resistance index. Expression of HOXB4, TCF4 and CCND2 in each primary AML patient sample was normalized to the geometric mean of all samples. The sum of these relative expression values of all three genes were added up to a resistance index, which was plotted in comparison to the JQ1 IC50 of each sensitive (IC50 < 200 nM, blue dots) and resistant (IC50 > 500 nM, red dots) AML patient sample. Two-tailed Pearson correlation coefficient r and P value are shown.

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