Extended Data Figure 3 : Gene expression in human and mouse.

From: Whole‐genome sequencing identifies EN1 as a determinant of bone density and fracture

Extended Data Figure 3

a, Quantification of Dock8 expression and its temporal pattern through RNA‐seq in cultured calvarial murine osteoblasts across day 2 through to day 18 of osteoblast development. Shown for comparison is Bglap, which encodes osteocalcin, a critical protein in osteoblasts. b, Quantification of expression of genome‐wide significant genes and their temporal pattern through RNA‐seq in cultured calvarial murine osteoblasts across day 2 through to day 18 of osteoblast development. c, Expression of EN1 mRNA in human cells presented as per cent of GAPDH mRNA. d, Expression of En1 in control and sdEn1 mice in purified osteoblast culture. For osteoblast marker gene expression, total mRNAs were purified from osteoblast cultures at day 10 and measured using quantitative real‐time PCR. mRNA levels were normalized relative to GAPDH mRNA. e. Real‐time PCR expression of control and sdEn1 as compared to 18S mRNA in whole vertebral bone extract. All data are shown as mean ± s.e.m. Significance computed by Student’s unpaired t‐test.