Extended Data Figure 5 : STOMAGEN overexpression on stomatal development in tmm hypocotyl epidermis with combinatorial loss-of-function in ER-family genes: a complete set.

From: Competitive binding of antagonistic peptides fine-tunes stomatal patterning

Extended Data Figure 5

ar, Representative confocal microscopy images of hypocotyl epidermis from 10-day-old light-grown transgenic seedlings of the following genotypes, each carrying Est::STOMAGEN: wild-type (wt) (a, b); tmm (c, d); tmm er (e, f); tmm erl2 (g, h); tmm erl1 (i, j); tmm er erl2 (k, l); tmm erl1 erl2 (m, n); tmm er erl1 (o, p); and tmm er erl1 erl2 (q, r). A control, uninduced phenotype (a, c, e, g, i, k, m, o, q); iSTOMAGEN (b, d, f, h, j, l, n, p, r). iSTOMAGEN results in arrested stomatal precursor cells (asterisks) and stomatal-lineage ground cells (SLGCs; brackets) in tmm hypocotyls (d). iSTOMAGEN triggers entry divisions in tmm er and tmm erl2 (f, h; brackets), and exaggerate the SLGC clusters in tmm er erl2 (k, l; brackets). Images were taken under the same magnification. Scale bar, 30 μm. n = 19 (a); n = 19 (b); n = 20 (c); n = 20 (d); n = 19 (e); n = 22 (f); n = 20 (g); n = 17 (h); n = 18 (i); n = 19 (j); n = 19 (k), n = 21 (l); n = 17 (m); n = 20 (n); n = 19 (o); n = 21 (p); n = 20 (q); n = 20 (r). s, t, Stomatal index and SLGC index. s, ***P < 0.0001; **P < 0.01; *P < 0.5 (Wilcoxon rank sum test). NS, not significant. 0, no stomata or SLGC observed; n = 15. Total number of stomata and SLGCs counted; tmm non-induced, 0 and 0; induced, 0 and 211; tmm er non-induced, 0 and 0; induced, 0 and 308; tmm erl2 non-induced, 0 and 32; induced, 0 and 171; tmm erl1 non-induced, 58 and 116; induced, 142 and 138; tmm er erl2 non-induced, 0 and 270; induced, 10 and 676; tmm er erl1 non-induced, 422 and 283; induced, 817 and 422; tmm erl1 erl2 non-induced, 72 and 83; induced, 163 and 97; tmm er erl1 erl2 non-induced, 1,229 and 295; induced, 1,068 and 222. n = 15 for all genotypes (s, t).