Extended Data Figure 4 : Characterization of Notch pathway components in the steady-state murine tracheal epithelium.

From: Parent stem cells can serve as niches for their daughter cells

Extended Data Figure 4

a, Relative mRNA expression of Notch1, Notch3 and Notch4 assessed by qRT–PCR in pure sorted populations of airway epithelial cells (n = 3 mice). Relative expression is normalized to baseline transcript levels in secretory progenitor cells. b, Immunostaining for N1ICD (red) in combination with the basal cell marker p63 (top panel), the secretory cell marker SSEA-1 (middle panel) and the ciliated cell marker FOXJ1 (bottom panel) (green). c, Immunostaining for N3ICD (red) in combination with the basal cell marker podoplanin (PDPN) (top panel), the secretory cell marker SSEA-1 (middle panel) and the ciliated cell marker FOXJ1 (bottom panel) (green). d, Relative mRNA expression of Hes1, Hey1 and HeyL assessed by qRT–PCR in pure sorted populations of airway epithelial cells (n = 3 mice). Relative expression is normalized to baseline transcript levels in secretory progenitor cells. n = biological replicates/condition. **P < 0.01; ***P < 0.001. nd indicates lack of detection. Data shown in the graphs are means ± s.e.m. Nuclei stained with DAPI (blue). White arrowheads point to double positive cells. Scale bar, 20 μm.