Extended Data Figure 9: Polarization of cortical myosin II in anaphase does not depend on PP1 phosphatase. | Nature

Extended Data Figure 9: Polarization of cortical myosin II in anaphase does not depend on PP1 phosphatase.

From: Kinetochore-localized PP1–Sds22 couples chromosome segregation to polar relaxation

Extended Data Figure 9

ac, Data show the impact of PP1-87B silencing (16 cells from four animals) on myosin repolarization during anaphase onset relative to a control (12 cells from four animals). a, b, Stills of representative control (a) and PP1-87B-depleted (b) SOP cells in anaphase labelled for myosin II (Sqh–mCherry) and F-actin (GMA) (top), together with the corresponding kymographs showing the equator–pole (E–P) perimeter section during anaphase progression. c, Schematic and graph show length of actin and myosin-II domains along the E–P perimeter in control and PP1-87B RNAi SOP cells. Mean and s.d. are shown. Significance was assessed using a two-tailed unpaired t-test. P > 0.05 was deemed not significant (n.s.). Scale bar, 5 µm. n, number of cells. These data show that although PP1-87B controls the polarization of cortical actin in anaphase, it does not affect the timely accumulation of myosin II at the equator.

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