Extended Data Figure 8: Depletion of PP1-87B and Sds22 or expression of moesin(T559D)–GFP lead to severe shape defects in telophase cells. | Nature

Extended Data Figure 8: Depletion of PP1-87B and Sds22 or expression of moesin(T559D)–GFP lead to severe shape defects in telophase cells.

From: Kinetochore-localized PP1–Sds22 couples chromosome segregation to polar relaxation

Extended Data Figure 8

ad, Data show the impact of PP1-87B or Sds22 silencing and moesin(T559D) overexpression on telophase cell shape. a, Stills show representative telophase cells in control (1 from 32), PP1-87B RNAi (1 from 31) and Sds22 RNAi (1 from 27) backgrounds. Circularity of cells, C, is indicated. F-actin is labelled by Lifeact–GFP. b, Images show representative stills of telophase moesin–GFP (1 from 19) or moesin(T559D)–GFP (1 from 15) cells. Circularity of cells, C,is indicated. PIIa and PIIb are the cells that result from an asymmetric SOP division (in a and b; in each image, PIIa is the daughter cell on the left and PIIb is the daughter cell on the right.) c, Box plot of circularity of nascent cells at telophase in control, PP1-87B RNAi and Sds22 RNAi tissues. d, Box plot of circularity of nascent cells at telophase in moesin–GFP and moesin(T559D)–GFP-expressing tissues. In a and b, scale bar, 5 µm. Box-and-whisker plots show median and 10–90 percentiles. Significance was assessed using a two-tailed unpaired t-test.

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