Extended Data Figure 6: Depletion of Sds22 in human cells leads to impaired clearance of cortical actin. | Nature

Extended Data Figure 6: Depletion of Sds22 in human cells leads to impaired clearance of cortical actin.

From: Kinetochore-localized PP1–Sds22 couples chromosome segregation to polar relaxation

Extended Data Figure 6

a, Western blot showing depletion of Sds22 in HeLa cells through RNA interference. b, Control (representative of 18 cells) and Sds22 RNAi (representative of 11 cells) STLC-treated HeLa cells expressing Lifeact–GFP and H2B–mCherry before and after RO3306 treatment (which forces cells to exit mitosis). c, Scatter plot (median, red line) quantifying the minimal DNA-to-cortex distance after treatment with RO3306 in each case shown in b. d, Scatter plot (median, red line) showing cortical F-actin clearance below the DNA (as seen in Extended Data Fig. 4b); siRNA oligonucleotide no. 5 (seen in a) was used in experiments shown in bd and Fig. 3c, d. In b, scale bar, 5 µm. Significance was assessed using a two-tailed unpaired t-test.

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