Extended Data Figure 9 : Genome editing in human rsESCs.

From: An alternative pluripotent state confers interspecies chimaeric competency

Extended Data Figure 9

a, Schematic representation of the targeted mutagenesis approach employed in human ESCs and rsESCs by CRISPR/Cas9. b, Targeted mutagenesis efficiencies at the LRRK2 locus in human ESCs (treated with Y27632, 10 μM) and rsESCs. c, Schematic representation of the CRISPR/Cas9- or TALEN-mediated gene correction approaches in human ESCs and rsESCs containing a mutated GFP gene. d, GFP correction efficiencies in human ESCs (treated with Y27632, 10 μM) and rsESCs by CRISPR/Cas9 or TALEN. The y axis shows the gene correction efficiency, which was calculated as GFP-positive cells per 5 × 105 cells. Error bars indicate s.d. (n = 3, independent experiments).