Extended Data Figure 7 : Metabolic profiling of EpiSCs and rsEpiSCs.

From: An alternative pluripotent state confers interspecies chimaeric competency

Extended Data Figure 7

a, Basal respiration, b, c, Maximum respiration (b) and ATP production (c) were determined by calculating the average oxygen consumption rate (OCR) for each phase in EpiSCs and rsEpiSCs. d, e, f, Glycolysis (d), glycolytic capacity (e) and reserve glycolysis (f) were determined by calculating the average extracellular acidification rate (ECAR) for each phase in EpiSCs and rsEpiSCs. (Graph Pad Prism v5). g, Representative graph showing the oxygen consumption rate in response to oligomycin, FCCP and rotenone/antimycin of EpiSCs and rsEpiSCs (n = 4). h, Heat map of differentially expressed genes for mitochondrial complex COX and enzymes involved in glycolysis and the tricarboxylic acid cycle selected from the RNA-seq data set, P < 0.05. i, j, Volcano plots of hydrophilic (metabolomics) and hydrophobic (lipidomics) metabolites show broad changes in metabolite levels between EpiSCs and rsEpiSCs. Error bars indicate s.d.; t-test, **P < 0.01, *P < 0.05 (ag, n = 6, technical replicates).