Extended Data Figure 5 : Mechanistic studies of rsEpiSCs self-renewal.

From: An alternative pluripotent state confers interspecies chimaeric competency

Extended Data Figure 5

a, Phase-contrast images showing colony morphologies of rsEpiSCs after 4 days of indicated treatments. Left, N2B27 media alone; right, N2B27F/R1. b, Quantitative PCR analysis of expression of pluripotent (Oct4, Sox2 and Nanog), endodermal (Sox17 and Gsc), mesodermal (Eomes and Mixl1) and neuroectodermal (Sox1 and Pax6) markers after indicated treatments for 4 days in culture. Error bars indicate s.d. (n = 3, biological replicates). c, Schematic representation of how different signalling pathways are involved in promoting or inhibiting self-renewal of rsEpiSCs.