Extended Data Figure 4 : GW7647 improves anaemia in two mouse models of anaemia.

From: PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal

Extended Data Figure 4

a, Experimental scheme for PHZ- induced haemolytic anaemia, used also in Fig. 3a. Wild-type or Ppara−/− mice were pretreated with DMSO (control) or GW7647 (100 μg kg−1) for 3 days (days −3 to −1) before PHZ injection on day 0. Mice were subject to daily DMSO or GW7647 injections during days 0–6. Red arrows indicate days of blood sample collection. b, Ppara−/− mice were treated with DMSO or GW7647 and then injected with PHZ. Haemoglobin (HGB), red blood cell numbers (RBC) and haematocrit (HCT) were measured on the days indicated. Error bars represent mean ± s.d. from six mice. c, BFU-E and CFU-E colony-forming assays of spleen or bone marrow cells. Wild-type or Ppara−/− mice were treated with PHZ and DMSO (control) or GW7647 (100 μg kg−1) as described in earlier. *P < 0.05, Student’s t-test. Error bars represent mean ± s.d. from three independent experiments. d, Spleen and bone marrow cells were harvested. Representative flow cytometry analysis of spleen erythroblasts isolated from GW7647- or DMSO-treated wild-type mice at day 3 after PHZ injection. FSC-A, forward scatter area.