Extended Data Figure 5 : Circuit contributions to cool-PN responses.

From: Thermosensory processing in the Drosophila brain

Extended Data Figure 5

ad, Fast-cool-PNs. a, Mean firing rate for the fast-cool-PNs, ± s.e.m. Reproduced from Fig. 1b. b, Mean firing rate with synaptic inhibition blocked with picrotoxin and CGP54626 (n = 4–5). c, Mean firing rate in the Gr28b.d mutant (n = 5–10). d, Mean firing rate in the Gr28b.d mutant with inhibition blocked (n = 4–6). eh, Slow-cool-PNs. e, Mean firing rate for the slow-cool-PNs ±s.e.m. Reproduced from Fig. 1f. f, Mean firing rate with synaptic inhibition blocked with picrotoxin and CGP54626 (n = 4–5). g, Mean firing rate in the Gr28b.d mutant (n = 4–5). h, Mean firing rate in the Gr28b.d mutant with inhibition blocked (n = 4). In the Gr28b.d mutant, note that blocking inhibition produces only modest disinhibition in the cool-PNs. This contrasts with our results in the warm-PNs and warm–cool-PNs, where blocking inhibition in the mutant abolished all excitation evoked by the preferred stimulus for these neurons (Fig. 2 and Fig. 3). Genotypes are: ad, Gr28bMB03888;GMR95C02-Gal4,pJFRC2-10XUAS-IVS-mCD8::GFP and eh, Gr28bMB03888;GMR67D03-Gal4,pJFRC2-10XUAS-IVS-mCD8::GFP.