Extended Data Figure 6 : In situ hybridization (ISH) validates predicted presence or absence of gene expression in hRG and mRG.

From: Radial glia require PDGFD–PDGFRβ signalling in human but not mouse neocortex

Extended Data Figure 6

a, Pink box: human in situ probes for six genes predicted to be expressed by hRG but not mRG were generated and hybridized in GW15 human neocortical tissue to validate predicted hRG expression (human scale bar, 200 μm). Blue box (Eurexpress20: http://www.eurexpress.org/ee/): in situ hybridizations for 13 genes predicted to be expressed by hRG but not mRG reveal no expression by mRG in E14.5 mouse cortex. Green box: mouse in situ probes for three genes predicted to be expressed by hRG but not mRG were generated and revealed no expression by mRG (E13.5). Positive control expression in cells other than RG are labelled in red. b, Expression patterns of genes predicted to be expressed by mRG (that is, those with the highest values in Supplementary Table 3) are shown as further validation (blue (E14.5, Eurexpress20: http://www.eurexpress.org/ee/); orange (E14.5, GenePaint: http://www.genepaint.org); mouse scale bars, 500 μm). One other gene in the top 15, Cks2, is not shown, but has been validated previously30.