Extended Data Figure 5 : MapZ position depends on PG synthesis and FtsZ position depends on MapZ functionality.

From: MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae

Extended Data Figure 5

a, Localization of MapZ after inhibition of PG synthesis in wild-type pneumococcus. Microscopy images of GFP–MapZ in wild-type cells before (top), and 15 min after addition of vancomycin (middle) or norfloxacin (bottom). Vancomycin, which inhibits PG synthesis, impairs localization of GFP–MapZ, whereas norfloxacin, which inhibits topoisomerases, has no effect on MapZ septal localization. b, c, Localization of FtsZ–GFP in mapZΔcyto (b) and corresponding FtsZ–GFP ring positioning along the cell length normalized to 1 (c). d, e, Localization of FtsZ–GFP in mapZΔextra (d) and corresponding FtsZ–GFP ring positioning along the cell length normalized to 1 (e). Images are representative of experiments made in triplicate.