Extended Data Figure 2 : Prediction of MapZ topology.

From: MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae

Extended Data Figure 2

a, MapZ topology (that is, specification of the membrane spanning segments and their in/out orientation relative to the membrane) was predicted by five different topology algorithms (SCAMPI-seq, SACAMPI-msa, PRODIV-TMHMM, PRO-TMHMM and OCTOPUS) using TOPCONS (http://topcons.net). ZPRED (green line) predicts the distance to the membrane centre of each amino acid and ΔG scale (light blue) shows the predicted free energy of membrane insertion for a window of 21 amino acids centred around each position in the sequence. The transmembrane span is indicated in grey. Predictions of cytoplasmic and extracellular localizations are shown in red and dark blue, respectively. b, Wide-field microscopy images of cells producing the C-terminal fusion of MapZ with GFP. GFP fluorescence (right panel) and phase contrast images (left panel). Images are representative of experiments made in triplicate.