Extended Data Figure 9 : Interplay between MapZ and StkP and PhpP and conservation of MaZ in bacterial genomes.

From: MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae

Extended Data Figure 9

a, Simultaneous localization of GFP–StkP and RFP–MapZ in wild-type cells. Overlays between GFP (green), RFP (red) and phase contrast show that StkP locates at mid-cell while MapZ ring separation proceeds, as depicted in the summary diagram below. b, Dephosphorylation of MapZ by PhpP. MapZ cytoplasmic domain was phosphorylated by StkPKD and then incubated for various times (30 s to 10 min) with the protein phosphatase PhpP. MapZ dephosphorylation was analysed by autoradiography. c, Conservation analysis of mapZ homologues in 6,305 bacterial genomes. The left panel shows the taxonomy of the bacterial superkingdom. The right panel indicates the number of genera, the number of sequenced genomes, the number of genomes coding for MapZ homologous proteins and the percentage of genomes coding for MapZ homologous proteins. Images are representative of experiments made in triplicate.