Extended Data Figure 6 : FtsZ is mispositioned in ΔmapZ cells and colocalizes with PG synthesis.

From: MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae

Extended Data Figure 6

a, Time-lapse images of FtsZ–GFP (green) dynamics in ΔmapZ cells. FtsZ polymers fail to position correctly even in cells with normal shape (arrow 1), resulting in asymmetric cell division or cell lysis (arrow 2) (stills correspond to Supplementary Video 5). b, Microscopy images showing colocalization of PG synthesis revealed by pulse labelling with TDL (red) and mispositioned FtsZ–GFP structures (green) in ΔmapZ cells. Three fields of view from three independent experiments are shown. c, 3D-SIM and schematic of FtsZ dumbbells with histograms of the cell ratios with 1, 2 or 3 rings. The average number of rings per cell is shown. d, 3D-SIM image of DAPI-stained DNA and FtsZ–GFP in ΔmapZ cells. e, Localization of GFP–MapZΔ(1–41) in gfp-mapZΔ(1–41). f, Localization of FtsZ–GFP in mapZΔ(1–41). g, Corresponding FtsZ–GFP ring positioning along the cell length normalized to 1. h, i, Time-lapse images of FtsZ–GFP (green) dynamics in mapZΔ(1–41) (h) and mapZΔcyto (i) cells. FtsZ mispositioning, even in cells with normal shape leads to asymmetric cell division (arrow 1) or cell lysis (arrow 2). Images are representative of experiments made in triplicate.