Extended Data Figure 4 : Cells expressing the D-box and KEN box receptor mutants of CDC20 can degrade cyclin B1 in nocodazole.

From: The mitotic checkpoint complex binds a second CDC20 to inhibit active APC/C

Extended Data Figure 4

Cyclin B1–Venus degradation was analysed in siRNA CDC20-treated cells rescued with siRNA-resistant versions of 3×Flag–CDC20, wild-type, or ΔDR, or ΔKR mutants, in the presence of nocodazole (0.33 µM). The fluorescence of individual cells was measured, the value at NEBD set to 1 and the mean ± s.e.m. for all cells plotted. n, number of cells analysed from at least two independent experiments.