Extended Data Figure 10 : Model for how the MCC could disseminate the ‘wait anaphase’ signal.

From: The mitotic checkpoint complex binds a second CDC20 to inhibit active APC/C

Extended Data Figure 10

Unattached kinetochores catalyse MCC formation and the MCC disseminates the ‘wait anaphase’ signal through the cytoplasm (black arrows). When the MCC disassembles (blue arrows), this releases CDC20, which along with newly synthesized CDC20, can have two fates: to be recruited to unattached kinetochores and incorporated into the MCC, or to bind the APC/C to form APC/CCDC20. The MCC is able to inhibit both unbound CDC20 and CDC20 bound to the APC/C (red bars).