Extended Data Table 1: Primers for site-directed mutagenesis of hTIM-3 and hCEACAM1

From: CEACAM1 regulates TIM-3-mediated tolerance and exhaustion

Table 1
  1. a, hTIM-3 primers. b, hCEACAM1 primers. Mutations were chosen on the basis of orthologous human residues reported previously13 in mTIM-3 as involved in non-galectin-9 interactions with an unknown ligand (hTIM-3 residues 1, 4, 6, 10 and 11 in a); orthologous human residues in hCEACAM1 (hCEACAM1 residues 1, 4, 5 and 6 in b) predicted to be involved in mTIM-3 interactions on the basis of studies described in Extended Data Fig. 2g and Supplementary Information; natural allelic variants of amino acid residues in hTIM-3 (residues 3, 7 and 10 in a) and hCEACAM1 (1, 4 and 6 in b) described above or others (hTIM-3 residue 7 in a), which were extracted from human exomic databases. Amino acid residues predicted to be involved in hTIM-3 (IgV)–hCEACAM1 (IgV) interactions on the basis of X-ray crystallographic structural models (hCEACAM1 residues 2 and 3 in b) as described in Supplementary Information, and cysteine residues involved in intrachain disulphide bonds adjacent to the CC′ and FG loops of hTIM-3 (Cys 58–Cys 109, Cys 52–Cys 63 and Cys 38–Cys 110) as described in Supplementary Information. Asterisks represent annotated human natural single nucleotide polymorphisms.