Extended Data Figure 6 : CEACAM1 and TIM-3 cooperatively regulate inflammation and anti-tumour immunity.

From: CEACAM1 regulates TIM-3-mediated tolerance and exhaustion

Extended Data Figure 6

a, Representative haematoxylin and eosin staining of groups described in Fig. 3e. Scale bar, 50 μm. b, Flow cytometry for intracellular cytokine assessment of TNF-α expression from infiltrating CD4+ T cells from inflamed colonic lamina propria of Ceacam1/ Rag2/ recipients, 6 weeks after transfer with naive CD4+ CD44lo CD62Lhigh T cells from indicated genotypes. c, Anorectal prolapse of indicated genotypes. d, Representative haematoxylin and eosin staining of groups described in Fig. 3g. Scale bar, 50 μm. e, RNA expression defined by nanostring of lamina propria mononuclear cells in indicated groups (mean of n = 3 per group). f, Schematic overview of protocol for AOM/DSS colitis-associated cancer model. g, Representative haematoxylin and eosin staining of colon from wild-type mice in AOM/1.5% DSS model. Scale bar, 50 μm; h, Representative photograph of distal colons of wild-type mice (n = 3 per group, anorectal junction at left end) in AOM/1.5% DSS model. Vertical arrows show the sites for dissection of the polyps (black) and the vicinity of the polyps (red). i, Representative flow cytometry analyses on infiltrating lymphocytes of invading distal colonic polyps or from the vicinity of the polyps or from mesenteric lymph nodes for CD4+ and CD8+ T cells and expression of CEACAM1 and TIM-3 or PD-1 and TIM-3. Note that vicinity of polyps exhibit highest numbers of T cells with an exhausted phenotype. j, Summary of flow cytometry on infiltrating lymphocytes from invading distal colonic polyps or from vicinity of polyps and from mesenteric lymph nodes for CD4+ and CD8+ T cells expressing CEACAM1 and TIM-3 or PD-1 and TIM-3 (n = 3, median shown). k, Representative pathology in AOM/1.5% DSS model. Scale bar, 60 μm. HGD, high grade dysplasia. Representative of three independent experiments (ae, gk).