A relative shift in cloacal location repositions external genitalia in amniote evolution

Abstract

The move of vertebrates to a terrestrial lifestyle required major adaptations in their locomotory apparatus and reproductive organs. While the fin-to-limb transition has received considerable attention1,2, little is known about the developmental and evolutionary origins of external genitalia. Similarities in gene expression have been interpreted as a potential evolutionary link between the limb and genitals3,4,5,6; however, no underlying developmental mechanism has been identified. We re-examined this question using micro-computed tomography, lineage tracing in three amniote clades, and RNA-sequencing-based transcriptional profiling. Here we show that the developmental origin of external genitalia has shifted through evolution, and in some taxa limbs and genitals share a common primordium. In squamates, the genitalia develop directly from the budding hindlimbs, or the remnants thereof, whereas in mice the genital tubercle originates from the ventral and tail bud mesenchyme. The recruitment of different cell populations for genital outgrowth follows a change in the relative position of the cloaca, the genitalia organizing centre. Ectopic grafting of the cloaca demonstrates the conserved ability of different mesenchymal cells to respond to these genitalia-inducing signals. Our results support a limb-like developmental origin of external genitalia as the ancestral condition. Moreover, they suggest that a change in the relative position of the cloacal signalling centre during evolution has led to an altered developmental route for external genitalia in mammals, while preserving parts of the ancestral limb molecular circuitry owing to a common evolutionary origin.

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Figure 1: A relative positional shift of limbs, genitalia and the cloaca in squamates.
Figure 2: Differential developmental origins of external genitalia in amniotes.
Figure 3: Molecular architecture of limbs and external genitalia in lizards and mice.
Figure 4: The cloacal signalling centre can recruit different mesenchymal cell populations for the outgrowth of external genitalia.

Accession codes

Primary accessions

Gene Expression Omnibus

Data deposits

Sequencing data has been deposited in the Gene Expression Omnibus under accession number GSE60373.

Change history

  • 17 December 2014

    Ref. 48 was corrected.

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Acknowledgements

The authors thank D. Duboule, H. Kaessmann, A. Necsulea, B. Okaty, G. Rey and G. P. Wagner for discussions, M. A. de Bakker for the snake Tbx5 probe and A. M. Herrera and M. J. Cohn for discussing and sharing unpublished results. μCT scans were performed at the Center for Nanoscale Systems, Harvard University (supported by National Science Foundation award ECS-0335765) and at the Museum of Comparative Zoology. Next-generation sequencing was performed at the HMS Biopolymers Facility and computational analyses were run on the Orchestra Cluster, HMS Research Computing. P.T. was supported by post-doctoral fellowships from the Swiss National Science Foundation, EMBO and the Human Frontiers Science Program. A.C.G. was supported by a post-doctoral fellowship from the Swiss National Science Foundation. This work was supported by National Institutes of Health grant R37-HD032443 to C.J.T.

Author information

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Authors

Contributions

P.T., J.G. and C.J.T. conceived the project and designed the experiments. P.T. performed most experiments and computational analyses. E.S. prepared CT scans and helped with statistical analyses. T.J.S. helped with CT scans, Anolis husbandry and embryo collection. A.C.G. produced lentiviruses and A.C.A. helped with grafting experiments. J.K.H., O.P. and J.G. initiated snake analyses. O.P. contributed snake embryos. J.G. contributed to chick lineage tracing experiments. P.T., J.G. and C.J.T. wrote the paper, with comments from co-authors.

Corresponding authors

Correspondence to Jérôme Gros or Clifford J. Tabin.

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Competing interests

The authors declare no competing financial interests.

Extended data figures and tables

Extended Data Figure 1 Two separable ventral cell populations give rise to the murine genital tubercle.

a, b, Injection into the most distal ventral part of the embryo, the tail bud, marks cells posterior/ventral to the phallic part of the urethra (a, arrow; n = 7), whereas injection closer to the allantois, into the infra-umbilical mesenchyme, labels cells anterior/dorsal to the phallic part of the urethra (b, arrow; n = 4). Cells lining the peritoneal cavity are also marked (arrowheads), owing to accidental piercing of the coelom. gt, genital tubercle; ur, urethra. Scale bars, 200 μm.

Extended Data Figure 2 The squamate hemipenis mesenchyme initiates with limb-like cellular dynamics from the coelomic epithelium through an EMT.

a, Injection of GFP-expressing lentiviruses into the coelom of chicken embryos at HH14 labels cells emerging from the epithelium that contribute to the hindlimb mesenchyme (arrowhead). b, In lizards, labelled cells leaving the coelomic epithelium contribute to the hemipenis mesenchyme (arrowheads). c, Dorsal view of the hindlimb region of an E10.0 mouse embryo. d, Transversal section of a limb bud, showing EMT of the coelomic epithelium (diffuse laminin staining, open arrowhead), as cells contribute to the limb-bud mesenchyme. e, Dorsal view of the budding hemipenis of a snake embryo, 1 day after egg deposition. f, Transversal section of the hemipenis region. The basement membrane of the coelomic epithelium is breaking down (open arrowhead), while it is intact for both the nephric duct and the surface ectoderm (arrowheads). go, Expression of genitalia and limb genes during hemipenis initation. gi, Tbx4 is expressed early (h, arrow) and late during hemipenis initiation, in both the coelomic epithelium (i, arrowhead) and the hemipenis mesenchyme (i, arrow). jl, Tbx5 is only expressed later, in the mesenchyme (l, arrow), but is absent from the coelomic epithelium (l, open arrowhead). mo, Limb marker gene Lhx9 (see also Fig. 4e) is absent from both epithelium (o, open arrowhead) and mesenchyme (o, open arrow), but can be detected in dI1 neurons (o, asterisk). All gene expression was assessed in at least n = 3 samples. cl, cloaca; co, coelom; hp, hemipenis; lb, limb; nd, nephric duct. Scale bars, 50 μm.

Extended Data Figure 3 Heat maps of Pearson’s and Spearman’s rank correlation coefficients and cluster analysis of whole-transcriptome data.

a, b, Hierarchical clustering on pairwise correlation coefficients for whole-transcriptome data from anole (a) and mouse (b) samples. Numbers at nodes represent approximately unbiased P values obtained by multiscale bootstrap resampling. Sample identifiers: a, anole; m, mouse; GT, genital tubercle; HP, hemipenis; LB, limb; e, early; l, late.

Extended Data Figure 4 Heat maps of Pearson’s and Spearman’s rank correlation coefficients and cluster analysis of transcription factor and signalling pathway data.

a, b, Hierarchical clustering on pairwise correlation coefficients of transcription factor (TF) and signalling pathway data from anole (a) and mouse (b) samples. Numbers at nodes represent approximately unbiased P values obtained by multiscale bootstrap resampling. Sample identifiers: a, anole; m, mouse; GT, genital tubercle; HP, hemipenis; LB, limb; e, early; l, late.

Extended Data Figure 5 Heterotopic grafting of the cloacal signalling centre leads to ectopic outgrowths and genitalia-like transcriptional changes.

ac, Schematics and close-up images of the cloacal grafting procedure. a, The cloaca of a stage HH17–19 GFP-transgenic chicken embryo (red rectangle) is transplanted into the proximal-ventral portion of the limb of a wild-type embryo. b, c, Only the ventral-most part of the cloaca, including the cloacal membrane, is dissected out (b, red box), and subsequently cleared of excess mesenchymal cells attached to the SHH-expressing endoderm (c, red outline). dg, Grafting of beads soaked in SHH and FGF can induce ectopic outgrowths on both limbs (e; n = 6/48) and tail (g; n = 3/31). hk, Ectopic expression of genital markers GATA2 (h, i, arrowheads) and RUNX1 (j, k, arrowhead) in limb buds, following cloaca-to-limb grafts. ln, Ectopic expression of genital marker GATA2 (m, arrowheads) and RUNX1 (n, arrowheads) in the tail region, following cloaca-to-tail grafts. All gene expression was assessed in at least n = 3 samples. al, allantois; cl, cloaca; lb, limb. Scale bar, 200 μm.

Extended Data Figure 6 Pairwise differential expression analysis of limb and genitalia transcriptomes.

ad, Smear plot visualization of differential expression analyses of early anole (a), late anole (b), early mouse (c) and late mouse (d) limb versus genitalia transcriptomes. Genes used for the Venn diagram in Fig. 4d (|log2(fold change)| > 1.5; P value < 0.05) are highlighted in red, core 25 marker genes (see Fig. 4e and text) are highlighted and labelled in blue. CPM, counts per million; FC, fold change. e, f, Heat map of Z-score-normalized expression values for all genes fulfilling Venn diagram criteria (n = 2,003), for anole (e) and mouse (f) data. Row-based hierarchical clustering was used; core 25 marker genes are indicated on the right.

Extended Data Figure 7 Comparative marker gene expression analysis in mouse and squamate embryos.

ar, Genitalia markers Isl1 (ad), Runx1 (e, f), Gata2 (gj), Eya4 (k, l), Tbx5 (mp) and Dkk2 (q, r). Gata2 only becomes visibly expressed at the later stages of house snake hemipenis development (j, inset). sa′, Limb markers Lhx9 (sv), Tbx18 (w, x) and Lmx1b (ya′). All gene expression was assessed in at least n = 3 samples. cl: cloaca; gt: genital tubercle; hp: hemipenis; lb: limb. Scale bar, 200 μm.

Extended Data Figure 8 Pairwise differential expression analysis of tail bud and genitalia transcriptomes.

a, b, Smear plot visualization of differential expression analyses of early anole (a) and early mouse (b) tail bud versus genitalia transcriptomes. Genes used as input for the Venn diagram in c (|log2(fold change)| > 1.5; P value < 0.05) are highlighted in red, overlapping 257 marker genes are highlighted in blue. Top 25 genes in the two species, based on logCPM (counts per million) and logFC (fold change), are labelled. c, Venn diagram showing overlap of pairwise differential expression analysis results (log(fold change) > 1.5, P value < 0.05) of tail bud versus genital tissues for early budding stages in both anole and mouse.

Supplementary information

Supplementary Table 1

This file contains a list of orthologous genes between mouse and anole employed for GO-term analysis. Genes are ordered according to their absolute loading value from principal component analysis (see Fig. 3e), for principal component 2 (PC2), and top 500 genes (in bold, see Fig. 3f) were used for GO-term enrichment analysis. (PDF 3748 kb)

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Tschopp, P., Sherratt, E., Sanger, T. et al. A relative shift in cloacal location repositions external genitalia in amniote evolution. Nature 516, 391–394 (2014). https://doi.org/10.1038/nature13819

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