Extended Data Figure 4: Proliferation status of retinal cells other than cones 15 days after shRB1 transduction of intact FW19 retinas. | Nature

Extended Data Figure 4: Proliferation status of retinal cells other than cones 15 days after shRB1 transduction of intact FW19 retinas.

From: Rb suppresses human cone-precursor-derived retinoblastoma tumours

Extended Data Figure 4

ad, Combined transduction with pLKO-shRB1-733 and -737. a, Ki67 not detected in NRL+ or rhodopsin+ rod photoreceptors or in calbindin+ horizontal cells. b, Ki67 detected in PAX6lo, nestin+ RPCs (white arrows) but not in PAX6hi, nestin horizontal, amacrine or ganglion cells (yellow arrows). c, Ki67 detected in CHX10+, CRX RPCs (white arrows) but not in CHX10+, CRX+ bipolar cells (yellow arrows). d, Percentage of cells co-expressing Ki67 and retinal cell markers. eh, Transduction with YFP-marked pLKO-YFP-shRB1-733. e, Ki67 detected in YFP+, L/M-opsin+ or YFP+, cone arrestin+ cone precursors (white arrows) and in an undefined YFP cell (yellow arrow). f, Ki67 not detected in YFP+, calbindin+ horizontal cells, YFP+, syntaxin+ or YFP+, PAX6+ amacrine cells, or in YFP+, NRL+ rod precursors. g, Ki67 detected (white arrows) or not detected (yellow arrows) in YFP+, nestin+ RPCs or glia, or in YFP+, CHX10+ RPCs or bipolar cells. h, Proportion of Ki67+ cells co-expressing YFP and retinal markers after transduction with pLKO-YFP-shRB1-733 or scrambled control. Values and error bars are mean and s.d. of triplicate assays. Scale bars, 20 μm. Analyses in ad and in eh represent two independent experiments. All immunostaining was performed at least twice.

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