Extended Data Figure 7: Characterization of Rb/p130-depleted retinoblastoma-like cells. | Nature

Extended Data Figure 7: Characterization of Rb/p130-depleted retinoblastoma-like cells.

From: Rb suppresses human cone-precursor-derived retinoblastoma tumours

Extended Data Figure 7

a, Similar appearance of Rb/p130-depleted cones and early passage retinoblastoma cells. Scale bar, 40 μm. b, c, DNA copy number of shRNA vectors (b) or selected genes (c) in cell lines derived from Rb/p130-depleted cone precursors (Cone1, Cone2, Cone5) or from Rb/p130-depleted unsorted retinal cells (All3, All4), in Rb-depleted unsorted retinal cells 4 days after transduction (All-RB1-KD-d4), in Y79 cells, or in FW21 retina (normal) (n = 6). All cell lines retained RB1 and p130 shRNA vectors and lacked RB1 or p130 copy number alterations. The Y79 MYCN copy number (78) is not shown (asterisk). dg, qPCR gene expression analyses in the indicated cell lines relative to cones transduced with scrambled control or FW21 retina (n = 6). d, All cell lines had diminished RB1 and p130 expression. eg, Altered expression of cell-cycle-related (e), cone-related (f) and apoptosis-related (g) genes. h, SNP-array analysis of two Rb/p130-depleted cone precursor cell lines (1, 2), revealing no megabase-size loss of heterozygosity (LOH) or copy number alterations (CNA). Data are representative of at least two analyses (bg) or analyses of two cell lines (h). Values and error bars are mean and s.d. of triplicate assays.

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