Extended Data Figure 3 : Placing Pstpip2cmo mice on a HFD does not cause abnormal weight gain, intestinal inflammation or extraintestinal translocation of commensal bacteria.

From: Dietary modulation of the microbiome affects autoinflammatory disease

Extended Data Figure 3

a, b, Wild-type BALB/cJ and Pstpip2cmo mice were fed ad libidum a LFD or a HFD. Body weight was measured in age-matched female (a) and male (b) mice at 12–16 weeks of age. Each point represents an individual mouse and the line represents the mean ± s.e.m. Data were combined from three independent experiments. ce, Colon length (c), colitis score based on rectal bleeding and stool consistency (d) and representative haematoxylin and eosin-stained sections (original magnification, ×20) (e) of the intestinal tract of LFD- and HFD-fed Pstpip2cmo mice aged 14–18 weeks. f, Presence of commensal bacteria in the spleen, liver, mesenteric lymph nodes and bone of wild-type and diseased LFD-fed Pstpip2cmo mice was evaluated by Gram staining and 16S rDNA qPCR analysis of eubacteria.