Extended Data Figure 1 : Placing Pstpip2cmo mice on a high-fat and cholesterol diet limits the development of inflammatory bone disease.

From: Dietary modulation of the microbiome affects autoinflammatory disease

Extended Data Figure 1

ae, Wild-type and Pstpip2cmo mutant mice were fed a LFD or HFD. Representative hind paw images (a) and representative pictures of popliteal lymph nodes (b) from wild-type, LFD Pstpip2cmo and HFD Pstpip2cmo mice at 12–14 weeks of age. c, d, Haematoxylin and eosin staining (original magnification, ×20) (c) and pathology scores (d) of tail samples from 12–14-week-old wild-type, LFD Pstpip2cmo and HFD Pstpip2cmo mice. Pathology scores were assigned in a blinded fashion by a veterinary pathologist based on the extent and severity of inflammation, osteolysis and osteogenesis. e, Representative immunostaining of neutrophils and macrophages in hind paw sections from 14–18-week-old Pstpip2cmo mice that were fed either a LFD or a HFD (original magnification, ×60). ***P < 0.001; Student’s t-test.