Extended Data Figure 9 : Depletion of neutrophils in anti-Ly6G treated Pstpip2cmo mutant mice.

From: Dietary modulation of the microbiome affects autoinflammatory disease

Extended Data Figure 9

Wild-type and Pstpip2cmo mice received either PBS or 500 μg per mouse anti-Ly6G antibody (clone IA8) by intraperitoneal injection every 4–5 days starting at 6 weeks of age. ac, Two weeks after the first anti-Ly6G treatment, FACS analysis was performed on peripheral blood leukocytes (PBLs). a, Representative FACS plots of Gr-1 and CD11b expression on CD45.2+ gated cells. b, Enumeration of CD45.2+ Gr-1hi CD11b+ neutrophils in equal volumes of peripheral blood. c, Numbers of T cells (CD45.2+ TCRβ+), CD45.2+ Gr-1CD11b+ monocytes/macrophages and CD45.2+ Gr-1int CD11b+ cells in equal volumes of peripheral blood. Each point represents an individual mouse and the line represents the mean ± s.e.m. ***P < 0.001; Student’s t-test.