Extended Data Figure 8 : Reduced pro-IL-1β expression and IL-1β maturation in neutrophils isolated from HFD-fed Pstpip2cmo mice.

From: Dietary modulation of the microbiome affects autoinflammatory disease

Extended Data Figure 8

a, Wild-type, Pstpip2cmo and Pstpip2cmo × Il1b−/− bone-marrow-derived macrophages were left untreated or were primed with LPS for 3 h followed by stimulation with ATP (30 min) or silica (12 h), and IL-1β processing was evaluated by western blot. Data are representative of three independent experiments. b, Western blotting for pro-IL-1β in untreated neutrophils that were purified from wild-type, LFD-fed Pstpip2cmo and HFD-fed Pstpip2cmo mice. Data are representative of two independent experiments. c, d, Neutrophils (c) or macrophages (d) from wild-type, LFD-fed Pstpip2cmo and HFD-fed Pstpip2cmo mice were left untreated, or primed with LPS for 3 h and then stimulated with ATP (30 min) or silica (12 h), and IL-1β processing was evaluated by western blotting. Data are representative of two independent experiments.