Figure 1: Resection in the right direction. | Nature

Figure 1: Resection in the right direction.

From: Making the cut

Figure 1

Cannavo and Cejka1 examined the role of the Mre11–Rad50–Xrs2 (MRX) enzyme complex and Sae2 protein in end resection, the first step in repairing double-stranded breaks in DNA. Using a protein block to mimic a natural DNA break, they found that MRX makes a nick in the DNA at 15 to 20 nucleotides (nt) from the 5′ end of the break, a process that is promoted by phosphorylated (P) Sae2. The nick creates an entry site for Mre11 to proceed back to the break end in the 3′ to 5′ direction, and for the Exo1 or Dna2 enzymes to cleave 5′ to 3′, extending the resected end to leave a 3′ single-stranded DNA tail.

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