Extended Data Figure 4 : Ret conditional knockout mice and analysis of haematopoietic stem cells.

From: The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function

Extended Data Figure 4

a, Ret conditional knockout. Scheme of the targeted Ret allele. b, E14.5 fetal liver HSCs from Retfl littermate controls (two animals) and Ret conditional knockout Vav1-iCre.RetΔ (three animals) and deleted bone marrow control (last column) were purified by flow cytometry. Efficient deletion of Ret in Vav1-iCre.RetΔ cells was determined by qPCR as fold increase relative to littermate control cells. c, Number of E14.5 LSKs and total fetal liver cells. Retfl n = 8; RetΔ n = 8. d, Scheme of competitive transplantation with RetΔ animals and littermate controls, relative to Fig. 2h. e, Percentage of donor CD45.2 cells in blood cell lineages 16 weeks after primary and secondary transplantation. Error bars, s.e.m. *, ** and ***, P values for Student’s t-test lower than 0.05, 0.01 and 0.001 respectively.