Extended Data Figure 2 : LSKs are affected by Ret deficiency and have reduced reconstitution capacity.

From: The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function

Extended Data Figure 2

a, E14.5 total fetal liver cells. WT n = 20; Ret−/− n = 18. b, Flow cytometry analysis of E14.5 Ret−/− and WT littermate control LSKs (top) and myeloid progenitors (bottom). c, Flow cytometry analysis of Annexin V+ cells in cultured LSK cells. d, Flow cytometry analysis of E14.5 Ret−/− and WT littermate control HSCs. e, Flow cytometry analysis of E14.5 Ret−/− and WT LinCD150+CD48 HSC cells. Mean fluorescence intensity of cKit was analysed. No statistically significant differences were found. f, LincKit+ cells were labelled with CMTMR. Percentage of LincKit+CMTMR+ cells in bone marrow and spleen 20 h after injection (n = 3). g, h, Percentage of donor CD45.2 cells in blood cell lineages 16 weeks after primary and secondary (red) transplantation, relative to Fig. 2b, d. i, Day 12 CFU-s. WT n = 10; Ret−/− n = 10. Error bars, s.e.m. *, ** and ***, P values for Student’s t-test lower than 0.05, 0.01 and 0.001 respectively.