Extended Data Figure 1 : Ret expression in haematopoietic progenitors and Ret ligand expression in the fetal and adult HSC environment.

From: The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function

Extended Data Figure 1

a, FACS-sorted E14.5 fetal liver myeloid progenitors and LSK were analysed by RT–qPCR. b, FACS-sorted HSCs from E14.5 fetal liver and adult bone marrow were analysed by RT–qPCR. c, FACS-sorted E14.5 fetal liver and adult bone marrow HSCs were analysed by confocal microscopy. d, E14.5 fetal liver TER119CD45CD31+ endothelial cells (EC), TER119CD45CD31cKit+ICAM-1 hepatocyte progenitor cells (HP) and TER119CD45CD31 cKitICAM-1+ mesenchymal cells (MC) were analysed by RT–qPCR (left); negative controls relative to Fig. 1c were analysed by confocal microscopy (right). e, Bone marrow TER119CD45CD31+Sca1+ endothelial cells (Endo), TER119CD45CD31Sca1CD51+ osteoblasts (Osteo) and TER119CD45CD31Sca1+CD51+ mesenchymal stem cells (MSC) were analysed by RT–qPCR (left) and by confocal microscopy (right). f, E14.5 fetal liver and adult bone marrow were analysed by confocal microscopy. Arrows, candidate LinCD150+CD48CD41 HSCs, relative to Fig. 1d, e. Figure shows negative controls for GFL staining. g, E15.5 gut tissue was analysed by confocal microscopy. White bar, 5 μm. Error bars, s.e.m. Housekeeping genes: Gapdh and Hprt1. ***P value for one-way ANOVA lower than 0.001.