Letter | Published:

Metastasis-suppressor transcript destabilization through TARBP2 binding of mRNA hairpins

Nature volume 513, pages 256260 (11 September 2014) | Download Citation

Abstract

Aberrant regulation of RNA stability has an important role in many disease states1,2. Deregulated post-transcriptional modulation, such as that governed by microRNAs targeting linear sequence elements in messenger RNAs, has been implicated in the progression of many cancer types3,4,5,6,7. A defining feature of RNA is its ability to fold into structures. However, the roles of structural mRNA elements in cancer progression remain unexplored. Here we performed an unbiased search for post-transcriptional modulators of mRNA stability in breast cancer by conducting whole-genome transcript stability measurements in poorly and highly metastatic isogenic human breast cancer lines. Using a computational framework that searches RNA sequence and structure space8, we discovered a family of GC-rich structural cis-regulatory RNA elements, termed sRSEs for structural RNA stability elements, which are significantly overrepresented in transcripts displaying reduced stability in highly metastatic cells. By integrating computational and biochemical approaches, we identified TARBP2, a double-stranded RNA-binding protein implicated in microRNA processing, as the trans factor that binds the sRSE family and similar structural elements—collectively termed TARBP2-binding structural elements (TBSEs)—in transcripts. TARBP2 is overexpressed in metastatic cells and metastatic human breast tumours and destabilizes transcripts containing TBSEs. Endogenous TARBP2 promotes metastatic cell invasion and colonization by destabilizing amyloid precursor protein (APP) and ZNF395 transcripts, two genes previously associated with Alzheimer’s and Huntington’s disease, respectively. We reveal these genes to be novel metastasis suppressor genes in breast cancer. The cleavage product of APP, extracellular amyloid-α peptide, directly suppresses invasion while ZNF395 transcriptionally represses a pro-metastatic gene expression program. The expression levels of TARBP2, APP and ZNF395 in human breast carcinomas support their experimentally uncovered roles in metastasis. Our findings establish a non-canonical and direct role for TARBP2 in mammalian gene expression regulation and reveal that regulated RNA destabilization through protein-mediated binding of mRNA structural elements can govern cancer progression.

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Accessions

Primary accessions

Gene Expression Omnibus

Data deposits

RNA-seq and microarray data have been deposited in the Gene Expression Omnibus under accession number GSE49649.

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Acknowledgements

We thank C. Alarcon, N. Halberg, N. Pencheva, P. Furlow and A. Nguyen for technical assistance and comments on previous versions of this manuscript. We thank H. Lee for her help with orthotopic injections. We thank P. Oikonomou for providing the reporter constructs. We thank the Darnell laboratory for their input on the HITS-CLIP methodology. We are also grateful to M. McManus and Z. Mourelatos for providing Dicer-null cells. We thank C. Zhao, W. Zhang and S. Dewell of the Rockefeller Genomics Resource Center for assistance with next-generation RNA-sequencing and microarray profiling. H.G. is an Anderson Cancer Center Fellow at Rockefeller University. S.F.T. is a Department of Defense Era of Hope Scholar and a Department of Defense Breast Cancer Collaborative Scholars and Innovators Award recipient. S.T. was supported by the National Human Genome Research Institute (R01 HG003219). We also acknowledge the efforts of Expression Project for Oncology (ExpO) and International Genomics Consortium (IGC) in providing public access to breast cancer gene expression profiles and annotations.

Author information

Affiliations

  1. Laboratory of Systems Cancer Biology, Rockefeller University, 1230 York Avenue, New York, New York 10065, USA

    • Hani Goodarzi
    • , Steven Zhang
    • , Colin G. Buss
    • , Lisa Fish
    •  & Sohail F. Tavazoie
  2. Department of Biochemistry and Molecular Biophysics, and Department of Systems Biology, Columbia University, New York, New York 10032, USA

    • Saeed Tavazoie

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Contributions

H.G., S.T. and S.F.T. conceived the project and designed the experiments. S.F.T. and S.T. supervised all research. H.G., S.Z., S.T. and S.F.T. wrote the manuscript. H.G., S.Z., C.G.B. and L.F. performed the experiments. S.Z. performed cancer cell invasion and reporter assays. L.F. and H.G. performed the HITS-CLIP experiment. C.G.B. and H.G. performed lung colonization assays. H.G. performed the analyses.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Saeed Tavazoie or Sohail F. Tavazoie.

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https://doi.org/10.1038/nature13466

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