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Venkatesh et al. reply

Nature volume 511, pages E9E10 (10 July 2014) | Download Citation


Replying to J. M. Dijkstra Nature 511, (2014)

In the accompanying Comment1, Dijkstra has identified gene models potentially encoding cytokines in regions of the Callorhinchus milii genome, whose syntenic counterparts in teleost and tetrapod genomes harbour interleukin genes. These genes were not predicted by our assembly and annotation pipeline2. TBLASTN searches of the genome assembly and RNA-sequencing (RNA-seq) transcriptomes from 10 tissues (including thymus and spleen) of the elephant shark, and RNA-seq transcriptomes from the thymus and spleen of nurse shark using human and teleost fish interleukins as query sequences, were also unable to pick up these genes; although we readily detected other cytokine genes in the same family (for example, interleukin-7 (IL-7) and IL-15). Below are our comments on the candidate genes identified by Dijkstra.

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Author information


  1. Comparative Genomics Laboratory, Institute of Molecular and Cell Biology, A*STAR, Biopolis, Singapore 138673.

    • Byrappa Venkatesh
    •  & Alison P. Lee
  2. Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228.

    • Byrappa Venkatesh
  3. Department of Developmental Immunology, Max-Planck-Institute of Immunobiology and Epigenetics, Stuebeweg 51, 79108 Freiburg, Germany

    • Jeremy B. Swann
    •  & Thomas Boehm
  4. Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland 21201, USA

    • Yuko Ohta
    •  & Martin F. Flajnik
  5. Department of Pathology, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan

    • Masanori Kasahara
  6. The Genome Institute at Washington University, St. Louis, Missouri 63108, USA

    • Wesley C. Warren


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Correspondence to Byrappa Venkatesh or Wesley C. Warren.

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