Extended Data Figure 4: SDS–PAGE analysis of WT, ΔNegC, AutoN-2RA, ΔN-term and ΔC-term ACF complexes. | Nature

Extended Data Figure 4: SDS–PAGE analysis of WT, ΔNegC, AutoN-2RA, ΔN-term and ΔC-term ACF complexes.

From: Histone H4 tail mediates allosteric regulation of nucleosome remodelling by linker DNA

Extended Data Figure 4

a, WT Snf2h, ΔNegC Snf2h or AutoN-2RA Snf2h was co-expressed with Acf1-Flag in Sf9 insect cells and purified by affinity chromatography. b, ΔN-term Acf1 or ΔC-term Acf1 was co-expressed with Snf2h-Flag and purified by affinity chromatography. The presence of both Acf1 and Snf2h in each case indicated that WT SNF2h, ΔNegC SNF2h and AutoN-2RA SNF2h can all form complexes with Acf1 and that ΔN-term Acf1 and ΔC-term Acf1 can both form complexes with Snf2h.

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