Extended Data Figure 3: DNA linker-length sensing by ACF is quantitatively similar for mononucleosomes and dinucleosomes. | Nature

Extended Data Figure 3: DNA linker-length sensing by ACF is quantitatively similar for mononucleosomes and dinucleosomes.

From: Histone H4 tail mediates allosteric regulation of nucleosome remodelling by linker DNA

Extended Data Figure 3

a, The dinucleosomes contain a distal FRET-labelled nucleosome and a proximal unlabelled nucleosome connected by n bp of internucleosomal linker DNA. The flanking linker DNA is 3 bp and 40 bp on the side of the FRET-labelled and the unlabelled nucleosome, respectively. To facilitate the study of translocation of the FRET-labelled nucleosome, we placed 2-nt ssDNA gaps at the SHL ± two sites of the proximal nucleosome to prevent its repositioning. b, Comparison of translocation and pause-phase exit rates for mononucleosomes (filled bars) and dinucleosomes (hashed bars) with 40 bp and 78 bp linker lengths. [ACF] = 10 nM and [ATP] = 20 μM. Data are mean ± s.e.m. derived from at least 100 individual nucleosome remodelling traces from three independent experiments.

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