Extended Data Figure 1: Control experiments to test the effect of ATP and surface-anchoring on nucleosome remodelling. | Nature

Extended Data Figure 1: Control experiments to test the effect of ATP and surface-anchoring on nucleosome remodelling.

From: Histone H4 tail mediates allosteric regulation of nucleosome remodelling by linker DNA

Extended Data Figure 1

a, Ensemble remodelling time courses of [WT H4, 78 bp] nucleosomes by 10 nM ACF with 5 µM ATP (filled symbols) or without ATP (open symbols). Nucleosome translocation was monitored by the emission intensity of the FRET acceptor Cy5 under constant 532 nm illumination that excited the FRET donor Cy3. b, Comparison of the average remodelling kinetics for surface-anchored [WT H4, 40 bp] nucleosomes (measured by the single-molecule assay, more than 250 nucleosomes) and freely diffusing [WT H4, 40 bp] nucleosomes in solution (measured by the ensemble assay). [ACF] = 5 nM and [ATP] = 20 μM.

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