Extended Data Figure 4 : Sox2 DNA copy number assessed in mouse and human skin SCCs.

From: SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma

Extended Data Figure 4

a, Comparative genomic hybridization array performed on DNA from TECs compared to their corresponding germline bone marrow DNA of the same animal. Data are segmented and normalized in relation to the intensity of their neighbouring probes to detect with high confidence genomic regions that have been amplified or deleted42,43. Graph plot representing an overview of the aberrations found in chromosome 3 from a representative SCC. No amplification of the genomic region containing Sox2 (red box) is detected in TECs from invasive SCC. Horizontal blue lines represent the normalized log2 ratios of the DNA copy number of the different probes along the chromosome. Vertical bars indicate the regions with a certain probability of deletion (red) (P) or amplification (green) (1 − P). These analyses were performed on five different SCCs with similar results concerning the absence of Sox2 deletion. b, FISH experiment using a green-labelled SOX2 gene probe and an orange-labelled centromeric probe for chromosome 3 (CETN3) as reference probe against SOX2 (green) performed in actinic keratosis (AK), skin SCC and lung SCC human samples. These data show that, although the SOX2 gene is amplified in human lung SCC as previously described7, there is no SOX2 amplification in AK or in skin SCC. DAPI nuclear staining is represented in blue. Scale bars, 10 μm.