Extended Data Figure 10 : Functional characterization of skin SCCs following SOX2 deletion.

From: SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma

Extended Data Figure 10

a, Co-immunostaining for SOX2 protein (green) and CD34 (red) or K14 (purple) in control (Ctrl) (left) and Sox2 conditional knockout (cKO; right) SCCs after 2 weeks of tamoxifen administration. b, Co-immunostaining for K14 (green) and caspase 3 (red) in control (left) and Sox2 conditional knockout (right) SCCs after 2 weeks of tamoxifen administration. c, Quantification of the caspase-3-positive cells in the control and Sox2 conditional knockout SCCs showing the increase of apoptosis in the Sox2 conditional knockout SCCs (n = 5 SCCs from 4 different mice). d, Co-immunostaining for K14 (green) and PH3 (red) in control (left) and Sox2 conditional knockout (right) SCCs after 2 weeks of tamoxifen administration. e, Quantification of PH3-positive cells in control and Sox2 conditional knockout SCCs showing the decrease of proliferation in Sox2 conditional knockout SCCs (n = 5 SCCs from 4 different mice). Scale bars, 50 μm. Data represent the mean and s.e.m.