Extended Data Figure 8 : Molecular characterization of SOX2–GFP+ SCC TECs.

From: SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma

Extended Data Figure 8

a, Genetic strategy used to isolate SOX2–GFP-expressing TECs by FACS. b, Protocol used to induce skin carcinogenesis. c, FACS strategy used to isolate SOX2–GFP+ and SOX2–GFP TECs. d, Histograms summarizing the genes upregulated in SOX2–GFP+ TECs (the histograms show the mean and s.e.m. of microarray signals performed in duplicate). These data show that SOX2–GFP+ TECs of SCCs preferentially express genes involved in tumour stemness, proliferation/survival, cell adhesion/invasion, transcription and chromatin remodelling factors, DNA damage response and paraneoplasic hypercalcaemia. e, Venn diagram showing the overlap between the genes upregulated in SOX2–GFP+ TECs and the genes upregulated in wild-type E16 basal epidermal cells49,50 as compared to adult basal epidermal cells (fold change >3). The arrow indicates the hypergeometric P value of this overlap. f, FACS analysis of SOX2–GFP and CD133 within TECs from invasive SCCs. g, FACS quantification of the percentage of CD133+ cells in SOX2–GFP+ and SOX2–GFP SCC TECs (n = 5 SCCs). hj, Co-immunostaining of β4 integrin (white), SOX2–GFP (green) and SOX2 protein (h) or Igf2bp2 (i) or Itgα3 (red) (j), showing the co-expression of these markers by SOX2–GFP-expressing TECs. Epi, epithelium; Str, tumour stroma. Scale bars, 50 μm. Data represent the mean and s.e.m.