Focused specificity of intestinal TH17 cells towards commensal bacterial antigens

Abstract

T-helper-17 (TH17) cells have critical roles in mucosal defence and in autoimmune disease pathogenesis1,2,3. They are most abundant in the small intestine lamina propria, where their presence requires colonization of mice with microbiota4,5,6,7. Segmented filamentous bacteria (SFB) are sufficient to induce TH17 cells and to promote TH17-dependent autoimmune disease in animal models8,9,10,11,12,13,14. However, the specificity of TH17 cells, the mechanism of their induction by distinct bacteria, and the means by which they foster tissue-specific inflammation remain unknown. Here we show that the T-cell antigen receptor (TCR) repertoire of intestinal TH17 cells in SFB-colonized mice has minimal overlap with that of other intestinal CD4+ T cells and that most TH17 cells, but not other T cells, recognize antigens encoded by SFB. T cells with antigen receptors specific for SFB-encoded peptides differentiated into RORγt-expressing TH17 cells, even if SFB-colonized mice also harboured a strong TH1 cell inducer, Listeria monocytogenes, in their intestine. The match of T-cell effector function with antigen specificity is thus determined by the type of bacteria that produce the antigen. These findings have significant implications for understanding how commensal microbiota contribute to organ-specific autoimmunity and for developing novel mucosal vaccines.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Figure 1: Intestinal TH17 cells are specific for SFB- and other microbiota-derived antigens.
Figure 2: Most TH17 TCR hybridomas recognize SFB-unique proteins.
Figure 3: SFB-specific T cells become TH17 cells in the SILP.
Figure 4: TCR specificity for distinct luminal bacteria underlies divergent T helper cell differentiation in the SILP.

References

  1. 1

    Bettelli, E., Korn, T., Oukka, M. & Kuchroo, V. K. Induction and effector functions of TH17 cells. Nature 453, 1051–1057 (2008)

    CAS  ADS  Article  Google Scholar 

  2. 2

    McGeachy, M. J. & Cua, D. J. The link between IL-23 and Th17 cell-mediated immune pathologies. Semin. Immunol. 19, 372–376 (2007)

    CAS  Article  Google Scholar 

  3. 3

    Littman, D. R. & Rudensky, A. Y. Th17 and regulatory T cells in mediating and restraining inflammation. Cell 140, 845–858 (2010)

    CAS  Article  Google Scholar 

  4. 4

    Ivanov, I. I. et al. The orphan nuclear receptor RORγt directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell 126, 1121–1133 (2006)

    CAS  Article  Google Scholar 

  5. 5

    Ivanov, I. I. et al. Specific microbiota direct the differentiation of IL-17-producing T-helper cells in the mucosa of the small intestine. Cell Host Microbe 4, 337–349 (2008)

    CAS  Article  Google Scholar 

  6. 6

    Atarashi, K. et al. ATP drives lamina propria TH17 cell differentiation. Nature 455, 808–812 (2008)

    CAS  ADS  Article  Google Scholar 

  7. 7

    Atarashi, K. et al. Treg induction by a rationally selected mixture of Clostridia strains from the human microbiota. Nature 500, 232–236 (2013)

    CAS  ADS  Article  Google Scholar 

  8. 8

    Ivanov, I. I. et al. Induction of intestinal Th17 cells by segmented filamentous bacteria. Cell 139, 485–498 (2009)

    CAS  Article  Google Scholar 

  9. 9

    Gaboriau-Routhiau, V. et al. The key role of segmented filamentous bacteria in the coordinated maturation of gut helper T cell responses. Immunity 31, 677–689 (2009)

    CAS  Article  Google Scholar 

  10. 10

    Wu, H. J. et al. Gut-residing segmented filamentous bacteria drive autoimmune arthritis via T helper 17 cells. Immunity 32, 815–827 (2010)

    CAS  Article  Google Scholar 

  11. 11

    Lee, Y. K., Menezes, J. S., Umesaki, Y. & Mazmanian, S. K. Proinflammatory T-cell responses to gut microbiota promote experimental autoimmune encephalomyelitis. Proc. Natl Acad. Sci. USA 108 (Suppl 1). 4615–4622 (2011)

    CAS  ADS  Article  Google Scholar 

  12. 12

    Ivanov, I. I. & Honda, K. Intestinal commensal microbes as immune modulators. Cell Host Microbe 12, 496–508 (2012)

    CAS  Article  Google Scholar 

  13. 13

    Hooper, L. V., Littman, D. R. & Macpherson, A. J. Interactions between the microbiota and the immune system. Science 336, 1268–1273 (2012)

    CAS  ADS  Article  Google Scholar 

  14. 14

    Schnupf, P., Gaboriau-Routhiau, V. & Cerf-Bensussan, N. Host interactions with Segmented Filamentous Bacteria: An unusual trade-off that drives the post-natal maturation of the gut immune system. Semin. Immunol. 25, 342–351 (2013)

    CAS  Article  Google Scholar 

  15. 15

    Lochner, M. et al. Restricted microbiota and absence of cognate TCR antigen leads to an unbalanced generation of Th17 cells. J. Immunol. 186, 1531–1537 (2011)

    CAS  Article  Google Scholar 

  16. 16

    Awasthi, A. et al. Cutting edge: IL-23 receptor GFP reporter mice reveal distinct populations of IL-17-producing cells. J. Immunol. 182, 5904–5908 (2009)

    CAS  Article  Google Scholar 

  17. 17

    Ise, W. et al. CTLA-4 suppresses the pathogenicity of self antigen–specific T cells by cell-intrinsic and cell-extrinsic mechanisms. Nature Immunol. 11, 129–135 (2010)

    CAS  Article  Google Scholar 

  18. 18

    Sanderson, S., Campbell, D. J. & Shastri, N. Identification of a CD4+ T cell-stimulating antigen of pathogenic bacteria by expression cloning. J. Exp. Med. 182, 1751–1757 (1995)

    CAS  Article  Google Scholar 

  19. 19

    Sczesnak, A. et al. The genome of Th17 cell-inducing segmented filamentous bacteria reveals extensive auxotrophy and adaptations to the intestinal environment. Cell Host Microbe 10, 260–272 (2011)

    CAS  Article  Google Scholar 

  20. 20

    Prakash, T. et al. Complete genome sequences of rat and mouse segmented filamentous bacteria, a potent inducer of Th17 cell differentiation. Cell Host Microbe 10, 273–284 (2011)

    CAS  Article  Google Scholar 

  21. 21

    Kouskoff, V., Signorelli, K., Benoist, C. & Mathis, D. Cassette vectors directing expression of T cell receptor genes in transgenic mice. J. Immunol. Methods 180, 273–280 (1995)

    CAS  Article  Google Scholar 

  22. 22

    Kearney, E. R., Pape, K. A., Loh, D. Y. & Jenkins, M. K. Visualization of peptide-specific T cell immunity and peripheral tolerance induction in vivo. Immunity 1, 327–339 (1994)

    CAS  Article  Google Scholar 

  23. 23

    Moon, J. J. et al. Naive CD4+ T cell frequency varies for different epitopes and predicts repertoire diversity and response magnitude. Immunity 27, 203–213 (2007)

    CAS  Article  Google Scholar 

  24. 24

    Hsieh, C. S. et al. Development of TH1 CD4+ T cells through IL-12 produced by Listeria-induced macrophages. Science 260, 547–549 (1993)

    CAS  ADS  Article  Google Scholar 

  25. 25

    Hand, T. W. et al. Acute gastrointestinal infection induces long-lived microbiota-specific T cell responses. Science 337, 1553–1556 (2012)

    CAS  ADS  Article  Google Scholar 

  26. 26

    Scher, J. U. et al. Expansion of intestinal Prevotella copri correlates with enhanced susceptibility to arthritis. eLife 2, e01202 (2013)

    PubMed  PubMed Central  Google Scholar 

  27. 27

    Monk, I. R., Gahan, C. G. & Hill, C. Tools for functional postgenomic analysis of Listeria monocytogenes. Appl. Environ. Microbiol. 74, 3921–3934 (2008)

    CAS  Article  Google Scholar 

  28. 28

    Xayarath, B., Marquis, H., Port, G. C. & Freitag, N. E. Listeria monocytogenes CtaP is a multifunctional cysteine transport-associated protein required for bacterial pathogenesis. Mol. Microbiol. 74, 956–973 (2009)

    CAS  Article  Google Scholar 

  29. 29

    Alamyar, E., Giudicelli, V., Li, S., Duroux, P. & Lefranc, M. P. IMGT/HighV-QUEST: the IMGT(R) web portal for immunoglobulin (IG) or antibody and T cell receptor (TR) analysis from NGS high throughput and deep sequencing. Immunome Res. 8, 26 (2012)

    Google Scholar 

  30. 30

    Currier, J. R. & Robinson, M. A. Spectratype/immunoscope analysis of the expressed TCR repertoire. Curr. Protocols Immunol. Chapter 10, Unit 10.28. (2001)

  31. 31

    Langmead, B., Trapnell, C., Pop, M. & Salzberg, S. L. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 10, R25 (2009)

    Article  Google Scholar 

  32. 32

    Roberts, A., Pimentel, H., Trapnell, C. & Pachter, L. Identification of novel transcripts in annotated genomes using RNA-Seq. Bioinformatics 27, 2325–2329 (2011)

    CAS  Article  Google Scholar 

  33. 33

    Tubo, N. J. et al. Single naive CD4+ T cells from a diverse repertoire produce different effector cell types during infection. Cell 153, 785–796 (2013)

    CAS  Article  Google Scholar 

  34. 34

    Lauer, P., Chow, M. Y., Loessner, M. J., Portnoy, D. A. & Calendar, R. Construction, characterization, and use of two Listeria monocytogenes site-specific phage integration vectors. J. Bacteriol. 184, 4177–4186 (2002)

    CAS  Article  Google Scholar 

  35. 35

    Yu, N. Y. et al. PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes. Bioinformatics 26, 1608–1615 (2010)

    CAS  Article  Google Scholar 

  36. 36

    Yu, C. S., Chen, Y. C., Lu, C. H. & Hwang, J. K. Prediction of protein subcellular localization. Proteins 64, 643–651 (2006)

    CAS  Article  Google Scholar 

Download references

Acknowledgements

We thank S. Yong Kim for generating TCR transgenic mice, A. Viale for 454 pyrosequencing, R. Myers for RNA-seq, N. Freitag for providing the Listeria strain and expression vector, Y. Umesaki for SFB samples, and K. Murphy for providing the 58αβ hybridoma line. The Immune Monitoring Core New York University (NYU) is supported in part by grant UL1 TR00038 from the National Center for Advancing Translational Sciences and grant 5P30CA016087-32 from the National Cancer Institute; the NYU Histology Core is supported in part by grant 5P30CA016087-32 from the National Cancer Institute. M.K.J. was supported by grant R01 AI039614 from the NIH. Y.Y. was supported by the Arthritis National Research Foundation. M.X. is supported by the Irvington Institute fellowship program of the Cancer Research Institute. D.R.L. is a Howard Hughes Medical Institute Investigator.

Author information

Affiliations

Authors

Contributions

Y.Y. and D.R.L. designed the experiments and wrote the manuscript with input from the co-authors. Y.Y., M.B.T., M.X., C.N., A.C., X.L. and J.-J.L. performed most analyses. M.B.T. constructed TCR hybridomas. M.X. developed SFB-specific antibodies. M.G., H.X. and J.J.L. did TCR pyrosequencing analysis. J.L.L. and M.K.J. developed tetramers. F.A. and V.J.T. generated transgenic Listeria. A.S. performed RNA-seq analysis of SFB.

Corresponding author

Correspondence to Dan R. Littman.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Extended data figures and tables

Extended Data Figure 1 Stimulation of SILP TH17 cells requires intestinal microbiota antigen presentation.

a, Intestinal GFP+ CD4+ T cells from Il23rGFP/+ mice stimulated with faecal material from Jackson and Taconic mice in the presence of syngeneic splenic APCs. Forward scatter was evaluated after 2 days. b, TH17 cell activation by faecal material from SFB-monoassociated mice in the presence of APCs sufficient (WT) or deficient (KO) for MHC class II. c, Evaluation of potential activation of bystander CD4+ T cells upon stimulation with SFB antigen. SILP CD4+ T cells from mice with Jackson flora (Ly5.1) and Taconic flora (Ly5.2) were co-cultured or stimulated separately with APCs and SFB-monoassociated faecal material, and FSC was evaluated.

Extended Data Figure 2 Microbiota-dependent TCR usage bias among SILP TH17 cells.

a, SILP CD4+ T cells from Il23rGFP/+ mice were analysed for utilization of Vβs in TH17 cells versus non-TH17 cells. Ratios of the percentage of each TCR Vβ in GFP+ vs GFP cells are shown. Each symbol represents one mouse. b, Relative expression of Vβ14 and Vβ6 TCRs by SILP TH17 versus non-TH17 CD4+ T cells from Il23rGFP/+ mice. Left, representative FACS plots; Right, analysis of multiple animals. c, Specific enrichment of Vβ14 TCRs in CD4+ T cells expressing RORγt and IL-17A, but not FOXP3 or IFNγ. Left, representative FACS plots. Right, analysis of multiple animals. Each symbol represents one mouse. d, Correlation of Vβ14 enrichment in TH17 cells with the presence of specific commensal microbiota. B6 Jackson mice were housed alone or cohoused with B6 Taconic mice for two weeks. Left, representative FACS analyses. Right, analysis of multiple animals.

Extended Data Figure 3 TH17 TCR repertoire analysis by pyrosequencing.

a, Numbers of unique Vβ14 CDR3 sequences of individual SILP TH17 and non-TH17 samples. The sequences were normalized for numbers of cells and total reads. b, Preferential expansion of Vβ14+ clones in the TH17 compartment in the SILP. The proportions of the 10 most abundant Vβ14 CDR3 sequences from TH17 and non-TH17 cells from 8 mice are shown. c, TH17-non TH17 bias of unique Vβ14 CDR3 sequences in the SILP of multiple mice.

Extended Data Figure 4 Single-cell TCR cloning and TCR hybridoma screen.

a, Efficiency of single-cell Vβ14 cloning from SILP TH17 and non-TH17 cells of multiple mice. b, Distributions of unique Vβ14 sequences in TH17 and non-TH17 cells within the SILP. Each plot represents one mouse shown in a. y and x axes represent numbers of TH17 cells and non-TH17 cells for each unique Vβ14 sequence. Numbers of unique sequences are shown in coloured circles. c, Responses of TH17 and non-TH17 TCR hybridomas to small intestinal luminal contents from B6 Taconic and B6 Jackson mice. d, Stimulation of TH17 TCR hybridomas by SFB-monoassociated antigens in the presence of APCs sufficient (WT) or deficient (KO) for MHC class II.

Extended Data Figure 5 Identification of SFBNYU_003340 epitopes recognized by a subset of the TH17 TCR hybridomas.

a, Schematic representation of the antigen screen using a whole-genome shotgun SFB library. b, Stimulation of the 7B8 hybridoma by bacterial pool 3F12. c, Reactivity of 7B8 and four other TCR hybridomas with bacterial clone 3F12-E8. d, Diversity of the CDR3 sequences of TCRs specific for 3F12-E8. Note that they belong to different Vα subsets and have distinct Vβ14 CDR3 sequences. e, Responses of the 3F12-E8-specific TCR hybridomas to core epitopes encoded by minigenes expressed in E. coli.

Extended Data Figure 6 Identification of SFBNYU_004990 epitopes recognized by related TCRs.

a, Top, the distribution in TH17 and non-TH17 cells of four TCRs that share an identical TCRα chain. Bottom, amino acid alignment of the Vβ14 CDR3 sequences. The green box highlights the sequence differences. b, Stimulation of the 5A11 hybridoma by bacterial pool 2D10 in the SFB antigen screen. c, Responses of 4 TCR hybridomas, including a non-TH17 hybridoma, to bacterial clone 2D10-A10. d, Responses of the 2D10-A10-specific TCR hybridomas to core epitopes encoded by minigenes expressed in E. coli. e, TCR hybridoma responses to titrated synthetic peptide (IRWFGSSVQKV) in the presence of APCs.

Extended Data Figure 7 SFB epitopes recognized by diverse TH17 cell TCRs.

a, The epitopes recognized by the Vβ14+ TCR hybridomas stimulate only Vβ14+ TH17 cells from the SILP. TH17 cells sorted from Il23rGFP/+ mice were stimulated with indicated peptides (listed in d) in the presence of APCs. Left, representative IL-17A ELISPOT assay with triplicates. Right, normalized peptide-specific TH17 responses. Each dot represents one mouse. b, Polyclonal responses of Vβ14+ and Vβ14- SILP TH17 cells to SFB antigens. Representative FACS plots from five experiments are shown. c, Bioinformatics filtering approach to select candidate SFB epitopes. d, Summary of newly selected and the known A6 and A15 SFB peptides. e, IL-17A ELISPOT screen for indicated peptides using SILP TH17 cells sorted from SFB-colonized Il23rGFP/+ mice. The A6 peptide from SFBNYU_003340 and anti-CD3 served as positive controls. f, Vβ14 usage in TH17 cells specific for peptide N5. Left, representative IL-17A ELISPOT assay with triplicates for peptide N5, using Vβ14+ and Vβ14- SILP TH17 cells sorted from Il23rGFP/+ mice. Right, normalized N5-specific TH17 responses. Each dot represents one mouse.

Extended Data Figure 8 SFB-specific T cells become TH17 cells in SFB-colonized mice.

a, SFB-dependent 7B8Tg T cell accumulation in the SILP. 2 × 104 naive 7B8Tg T cells were transferred into congenic Ly5.1 recipient mice that were SFB-colonized or SFB-free. CD4+ T cells in the SILP were examined for donor and recipient isotype markers after 13 days. b, Top, strategy for co-transfer of congenic 1A2Tg and 5A11Tg T cells into SFB-colonized recipient mice. Bottom, FACS analysis of RORγt expression in host- and donor-derived CD4+ T cells in the SILP at 7 days after transfer. c, FACS analysis of transcription factors in host- and donor-derived SILP CD4+ T cells after transfer of naive 7B8Tg T cells as in a. d, FACS analysis of SILP T cells from Il23rGFP/+ mice, stained with I-Ab/3340-A6 tetramer and control tetramer (2W). e, FACS analysis of SILP T cells of B6 mice from colonies with different microbiota, stained with I-Ab/3340-A6 tetramer and intracellular RORγt antibody. f, Expansion of 7B8Tg T cells in mice colonized with Listeria monocytogenes expressing SFBNYU_003340. Top, immunofluorescence microscopic visualization of the expression of SFB protein by L. monocytogenes. Listeria-3340 and Listeria-empty were stained with anti-3340 rabbit polyclonal antibody. Red, anti-3340 antibody staining. Blue, DAPI staining. Bottom, naive Ly5.1+ 7B8Tg cells were transferred into congenic mice infected with Listeria-3340 or Listeria-empty. Seven days after transfer, donor-derived CD4+ T cells in the SILP were analysed by FACS.

Extended Data Figure 9 Transcription factor expression in SFB-specific and Listeria-specific T cells in co-infected mice.

Representative of data plotted in Fig. 4b. a, Experimental design for tracking both SFB- and Listeria- specific CD4+ T cells following intestinal colonization with both bacteria. Ly5.2 B6 mice were colonized with Listeria monocytogenes, SFB, or both bacteria, and 7B8Tg T cells from Ly5.1 mice were injected intravenously. Expression of TH1 and TH17 transcription factors in the SFB-specific 7B8Tg cells and LLO tetramer-specific recipient T cells was evaluated. b, Intracellular stain for RORγt. c, Intracellular stain for T-bet.

Extended Data Figure 10 SFB-specific TH17 cells are present in both SILP and large intestine lamina propria (LILP) of SFB-colonized mice.

T cells were stained with I-Ab/3340-A6 tetramer and antibody to intracellular RORγt. a, Representative FACS plots (gated on CD4+ T cells). b, Analysis of multiple animals. Left, per cent of tetramer-positive cells among total CD4+ T cells in each region of the intestine. Right, per cent of RORγt+ cells among the tetramer-positive cells. Each symbol represents cells from a separate animal.

Supplementary information

Supplementary Tables

This file contains Supplementary Table 1. (PDF 91 kb)

PowerPoint slides

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Yang, Y., Torchinsky, M., Gobert, M. et al. Focused specificity of intestinal TH17 cells towards commensal bacterial antigens. Nature 510, 152–156 (2014). https://doi.org/10.1038/nature13279

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing