Extended Data Figure 6: Gene expression in Rbpj mutant bone and lung endothelial cells. | Nature

Extended Data Figure 6: Gene expression in Rbpj mutant bone and lung endothelial cells.

From: Endothelial Notch activity promotes angiogenesis and osteogenesis in bone

Extended Data Figure 6

a, qPCR analysis of freshly isolated endothelial cells from control and RbpjiΔEC mutant lung or bone. Relative fold mRNA expression of Notch target genes such as Dll4, Jag1, Efnb2, EphB4, Hes1, Hey1, Hey2 is shown. Differently expressed genes are marked in red (n = 4 mice from 4 independent litters). Error bars, ± s.e.m. P values, two-tailed unpaired t-test. b, Quantitative PCR analysis of expression the Notch target gene Hes5 in sorted endothelial cells from 4-week-old wild-type bone and lung (left), and from RbpjiΔEC mutant and littermate control bone samples (right). Note very low expression of Hes5 in lung compared to bone endothelial cells and its reduction in the RbpjiΔEC mutant bone endothelium. Data represent relative fold mRNA expression (n = 4 mice from 4 independent litters). Error bars, ± s.e.m. P values, two-tailed unpaired t-test. c, qPCR results for transcripts encoding intercellular adhesion molecule 1 (Icam1), angiopoietin 2 (Angpt2), VE-cadherin (Cdh5), and CD31 (Pecam1). Data represent relative fold mRNA expression. Differently expressed genes are marked in red (n = 4 mice from 4 independent litters). Error bars, ± s.e.m. P values, two-tailed unpaired t-test. d, Relative fold mRNA expression of cyclin-dependent kinase inhibitor 2A (Cdkn2a), cyclin-dependent kinase inhibitor 1A (Cdkn1a), cyclin-dependent kinase inhibitor 1B (Cdkn1b), cyclin D1 (Ccnd1), cyclin-dependent kinase 2 (Cdk2), and cyclin-dependent kinase 4 (Cdk4) in control or RbpjiΔEC mutant lung and bone endothelial cells (n = 4 mice from 4 independent litters). Error bars, ± s.e.m. P values, two-tailed unpaired t-test.

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