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Biochemistry

Metal ghosts in the splicing machine

Chemical analysis of the spliceosome's active site reveals that it is the RNA components of this enzyme complex that coordinate the catalytic metal ions responsible for production of a spliced messenger RNA. See Article p.229

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Figure 1: Active sites of group II introns and the spliceosome.

References

  1. Sontheimer, E. J., Sun, S. & Piccirilli, J. A. Nature 388, 801–805 (1997).

    Article  ADS  CAS  Google Scholar 

  2. Fica, S. M. et al. Nature 503, 229–234 (2013).

    Article  ADS  CAS  Google Scholar 

  3. Fredericksen, J. K. & Piccirilli, J. A. Methods 49, 148–166 (2009).

    Article  Google Scholar 

  4. Yean, S. L. et al. Nature 408, 881–884 (2000).

    Article  ADS  CAS  Google Scholar 

  5. Pena, V., Rozov, A., Fabrizio, P., Lührmann, R. & Wahl, M. C. EMBO J. 27, 2929–2940 (2008).

    Article  CAS  Google Scholar 

  6. Marcia, M. & Pyle, A. M. Cell 151, 497–507 (2012).

    Article  CAS  Google Scholar 

  7. Galej, W. P., Oubridge, C., Newman, A. J. & Nagai, K. Nature 493, 638–643 (2013).

    Article  ADS  CAS  Google Scholar 

  8. Golas, M. M. et al. Mol. Cell 40, 927–938 (2010).

    Article  CAS  Google Scholar 

  9. Cech, T. R. Cell 44, 207–210 (1986).

    Article  CAS  Google Scholar 

  10. Nissen, P., Hansen, J., Ban, N., Moore, P. B. & Steitz, T. A. Science 289, 920–930 (2000).

    Article  ADS  CAS  Google Scholar 

Download references

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Correspondence to Scott A. Strobel.

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Strobel, S. Metal ghosts in the splicing machine. Nature 503, 201–202 (2013). https://doi.org/10.1038/nature12705

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