Abstract
Arising from B. D. Zoltowski et al. Nature 480, 396–399 (2011)10.1038/nature10618
Recently, we determined the X-ray crystal structure of full-length cryptochrome from Drosophila1. Here we report an improved model of the Drosophila cryptochrome (dCRY) structure that corrects errors in the original coordinates (Protein Data Bank (PDB) accession 3TVS). Further refinement of the structure, with automated rebuilding algorithms in Phenix2 followed by manual building, indicated that a model of dCRY could be produced with excellent refinement statistics without taking into account the non-merohedral twinning originally reported (Table 1).
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References
Zoltowski, B. D. et al. Structure of full-length Drosophila cryptochrome. Nature 480, 396–399 (2011)
Afonine, P. V., Grosse-Kunstieve, R. W. & Adams, P. D. The Phenix refinement framework. CCP4 Newslett. Number 42, contribution 8. (2005)
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The New York authors (B.D.Z., A.T.V., D.T., J.W., M.W.Y. and B.R.C.) carried out the work presented in ref. 1; the Manchester authors (C.L., A.R.J., N.S.S. and D.L.) identified errors in the original coordinates; together, both sets of authors carried out the further refinement of the structure presented here. C.L. initially identified errors in the original coordinates, and C.L. and D.L. performed initial model rebuilding and crystallographic analysis, which was verified and discussed with A.R.J. and N.S.S. The New York authors B.D.Z. and B.R.C. completed and verified the model rebuilding and crystallographic analysis. The New York authors A.T.V., D.T., J.W. and M.W.Y. contributed to structural analysis and verified the new structure against existing biochemical and biological data. B.R.C. and B.D.Z. wrote the communication with input from all other authors.
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Levy, C., Zoltowski, B., Jones, A. et al. Updated structure of Drosophila cryptochrome. Nature 495, E3–E4 (2013). https://doi.org/10.1038/nature11995
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DOI: https://doi.org/10.1038/nature11995
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