Genetic identification of C fibres that detect massage-like stroking of hairy skin in vivo

Abstract

Stroking of the skin produces pleasant sensations that can occur during social interactions with conspecifics, such as grooming1. Despite numerous physiological studies (reviewed in ref. 2), molecularly defined sensory neurons that detect pleasant stroking of hairy skin3,4 in vivo have not been reported. Previously, we identified a rare population of unmyelinated sensory neurons in mice that express the G-protein-coupled receptor MRGPRB4 (refs 5, 6). These neurons exclusively innervate hairy skin with large terminal arborizations7 that resemble the receptive fields of C-tactile (CT) afferents in humans8. Unlike other molecularly defined mechanosensory C-fibre subtypes9,10, MRGPRB4+ neurons could not be detectably activated by sensory stimulation of the skin ex vivo. Therefore, we developed a preparation for calcium imaging in the spinal projections of these neurons during stimulation of the periphery in intact mice. Here we show that MRGPRB4+ neurons are activated by massage-like stroking of hairy skin, but not by noxious punctate mechanical stimulation. By contrast, a different population of C fibres expressing MRGPRD11 was activated by pinching but not by stroking, consistent with previous physiological and behavioural data10,12. Pharmacogenetic activation of Mrgprb4-expressing neurons in freely behaving mice promoted conditioned place preference13, indicating that such activation is positively reinforcing and/or anxiolytic. These data open the way to understanding the function of MRGPRB4 neurons during natural behaviours, and provide a general approach to the functional characterization of genetically identified subsets of somatosensory neurons in vivo.

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Figure 1: In vivo calcium imaging in genetically defined subsets of primary sensory neurons.
Figure 2: Activation of MRGPRD fibres by pinching.
Figure 3: Activation of MRGPRB4 fibres by stroking.
Figure 4: Activation of MRGPRB4 neurons promotes conditioned place preference.

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Acknowledgements

We thank R. Robertson for programming and imaging data analysis; M. Walsh and T. Heitzman for the stimulus delivery system and associated electronics; M. Zelikowsky for help with statistical analysis of behavioural data; H. Inagaki for experimental advice and comments on the manuscript; S. Pease for help with generation of knock-in mice; N. Verduzco, K. Lee and R. Sauza for mice colony maintenance; M. Visel and J. Flannery for training in AAV8 preparation; A. Anderson and C. Pagan for initial experiments using their stereotaxic apparatus; J. Zhang and A. Basbaum for teaching the dorsal laminectomy and for comments on the manuscript; D. Davalos, K. Akassoglou and H. Johanssen for help with the in vivo imaging preparation; L. Lagnado and B. Odermatt for SypHy and SyGCamp2 plasmids; C. Shea and M. Martinez for technical assistance; H. Oates-Barker for laboratory management and G. Mancuso for administrative assistance. This work was supported by NIH grants 5PO1NS-48499 and 5R01 NS023476, and by fellowships from EMBO and the Human Frontiers Science Program (S.V.) and the Helen Hay Whitney Foundation (A.M.W.). D.J.A. is an Investigator of the Howard Hughes Medical Institute.

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S.V. carried out all imaging and behavioural experiments; A.M.W. helped to configure the two-photon imaging system and developed the light grid method; K.K.R. and H.R.K. carried out electrophysiological recordings in isolated skin-nerve preparations; D.J.A. participated in experimental design and data interpretation and wrote the manuscript together with S.V.

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Correspondence to David J. Anderson.

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This file contains Supplementary Figures 1-14, Supplementary Tables 1-3, a Supplementary Discussion, Supplementary Notes 1-10 and additional references. (PDF 4144 kb)

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Vrontou, S., Wong, A., Rau, K. et al. Genetic identification of C fibres that detect massage-like stroking of hairy skin in vivo . Nature 493, 669–673 (2013). https://doi.org/10.1038/nature11810

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