Defining the mode of tumour growth by clonal analysis

Abstract

Recent studies using the isolation of a subpopulation of tumour cells followed by their transplantation into immunodeficient mice provide evidence that certain tumours1,2, including squamous skin tumours3,4,5, contain cells with high clonogenic potential that have been referred to as cancer stem cells (CSCs). Until now, CSC properties have only been investigated by transplantation assays, and their existence in unperturbed tumour growth is unproven. Here we make use of clonal analysis of squamous skin tumours using genetic lineage tracing to unravel the mode of tumour growth in vivo in its native environment. To this end, we used a genetic labelling strategy that allows individual tumour cells to be marked and traced over time at different stages of tumour progression. Surprisingly, we found that the majority of labelled tumour cells in benign papilloma have only limited proliferative potential, whereas a fraction has the capacity to persist long term, giving rise to progeny that occupy a significant part of the tumour. As well as confirming the presence of two distinct proliferative cell compartments within the papilloma, mirroring the composition, hierarchy and fate behaviour of normal tissue, quantitative analysis of clonal fate data indicates that the more persistent population has stem-cell-like characteristics and cycles twice per day, whereas the second represents a slower cycling transient population that gives rise to terminally differentiated tumour cells. Such behaviour is shown to be consistent with double-labelling experiments and detailed clonal fate characteristics. By contrast, measurements of clone size and proliferative potential in invasive squamous cell carcinoma show a different pattern of behaviour, consistent with geometric expansion of a single CSC population with limited potential for terminal differentiation. This study presents the first experimental evidence for the existence of CSCs during unperturbed solid tumour growth.

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Figure 1: Genetic tracing of skin papilloma at clonal density.
Figure 2: Quantitative clonal analysis in skin papilloma.
Figure 3: Challenging the stem/progenitor cell model in papilloma.
Figure 4: Quantitative clonal analysis of squamous cell carcinoma.

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Acknowledgements

C.B. is investigator of WELBIO and chercheur qualifié of the FRS/FNRS. B.B. is chargé de recherche of the FRS/FNRS and G.D. is supported by the Brussels Region. This work was supported by the FNRS, the program d’excellence CIBLES of the Wallonia Region, a research grant from the Fondation Contre le Cancer, the ULB foundation and the fond Gaston Ithier, a starting grant of the European Research Council (ERC), and the EMBO Young Investigator Program. We thank F. Bollet-Quivogne and J.-M. Vanderwinden for their help with confocal imaging.

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C.B., G.D., B.B. and B.D.S. designed the experiments and performed data analysis. G.D. and B.B. performed most of the experiments. A.C. provided technical support. C.B. and B.D.S. wrote the manuscript.

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Correspondence to Benjamin D. Simons or Cédric Blanpain.

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The authors declare no competing financial interests.

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Driessens, G., Beck, B., Caauwe, A. et al. Defining the mode of tumour growth by clonal analysis. Nature 488, 527–530 (2012). https://doi.org/10.1038/nature11344

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