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Structure of the δ-opioid receptor bound to naltrindole

Abstract

The opioid receptor family comprises three members, the µ-, δ- and κ-opioid receptors, which respond to classical opioid alkaloids such as morphine and heroin as well as to endogenous peptide ligands like endorphins. They belong to the G-protein-coupled receptor (GPCR) superfamily, and are excellent therapeutic targets for pain control. The δ-opioid receptor (δ-OR) has a role in analgesia, as well as in other neurological functions that remain poorly understood1. The structures of the µ-OR and κ-OR have recently been solved2,3. Here we report the crystal structure of the mouse δ-OR, bound to the subtype-selective antagonist naltrindole. Together with the structures of the µ-OR and κ-OR, the δ-OR structure provides insights into conserved elements of opioid ligand recognition while also revealing structural features associated with ligand-subtype selectivity. The binding pocket of opioid receptors can be divided into two distinct regions. Whereas the lower part of this pocket is highly conserved among opioid receptors, the upper part contains divergent residues that confer subtype selectivity. This provides a structural explanation and validation for the ‘message–address’ model of opioid receptor pharmacology4,5, in which distinct ‘message’ (efficacy) and ‘address’ (selectivity) determinants are contained within a single ligand. Comparison of the address region of the δ-OR with other GPCRs reveals that this structural organization may be a more general phenomenon, extending to other GPCR families as well.

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Figure 1: Overall structure of the δ-OR.
Figure 2: Ligand-binding site of the δ-OR.
Figure 3: A conserved opioid ligand recognition mode.
Figure 4: The message–address hypothesis is reflected in opioid receptor structure.

Accession codes

Primary accessions

Protein Data Bank

Data deposits

Coordinates and structure factors for δ-OR–T4L are deposited in the Protein Data Bank under accession code 4EJ4.

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Acknowledgements

We thank R. Sunahara for helpful suggestions on opioid receptor pharmacology and biochemistry. We acknowledge support from INSERM (S.G.), the Stanford Medical Scientist Training Program (A.M.), the National Science Foundation (A.C.K.), National Institutes of Health grants NS028471 (B.K.K.) and DA031418 (B.K.K.), and from the Mathers Foundation (B.K.K. and W.I.W.).

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Authors

Contributions

A.M., A.C.K. and S.G. designed experiments, performed research and analysed data. T.S.K. and F.S.T. expressed and purified receptor. W.I.W. supervised diffraction data analysis and model refinement. A.M., A.C.K., S.G. and B.K.K. prepared the manuscript. S.G. and B.K.K. supervised the research.

Corresponding authors

Correspondence to Sébastien Granier or Brian K. Kobilka.

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The authors declare no competing financial interests.

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This file contains Supplementary Figures 1-9, Supplementary Table 1 and a Supplementary Bibliography. (PDF 13916 kb)

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Granier, S., Manglik, A., Kruse, A. et al. Structure of the δ-opioid receptor bound to naltrindole. Nature 485, 400–404 (2012). https://doi.org/10.1038/nature11111

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